经基因组原位杂交鉴定，BC1F4代中有披碱草的染色体组或染色体片段。
The genomic in situ hybridization showed that there are chromosomes or chromosome fragments of Elymus dahuricus in BC1F4.
端粒克隆、着丝粒克隆以及重叠群之间空缺的大小都经过粗线期染色体荧光原位杂交鉴定。
BAC clones corresponding to telomeres, as well as to the centromere position and the gap sizes between contigs, were determined by BAC-pachytene chromosome fluorescence in situ hybridization (FISH).
单株检测，RT - P CR分析，斑点杂交鉴定，确定此片段为花椰菜细胞质雄性不育系knxd612所特有。
Identified by individual plant testing, analysis of RT-PCR and dot blot, this fragment was only existed in CMS cauliflower knxd612.
研究结果表明，ISSR标记技术可以用来研究牡丹的杂交f 1代的鉴定以及品种间遗传与演化关系。
As a result, the ISSR marker technique can be used to carry out F1 hybrid identification and study the genetic relationship of tree peony cultivars.
对辣椒黄绿苗突变体的隐性叶色标记技术在纯度鉴定，提高杂交种子纯度以及在辣椒雄性不育中的作用及应用前景进行了探讨。
The leaf color mark technology of yellow-green seedling mutant is discussed in the function of purity identification, hybrid seed purity improvement and male sterility in hot pepper.
这两种方法不仅节约成本，且简单快速，大大降低了DNA的提取难度，适宜黄瓜杂交种子纯度鉴定。
Being simple and rapid, two methods could reduce the cost and make extraction of DNA very easily; thus they were suitable for the purity identification of Cucumber hybrid seeds.
生物芯片的应用是将探针固定于芯片上，利用核酸链间的分子杂交，鉴定dna和蛋白质的一种新技术。
The application of biochips is a new technique which fixes the probes on the biochips to appraise DNA and protein by the way of the molecule crosses between the chains of nucleic acids.
通过人工有性杂交、单株选择、系统选择、区试鉴定，育成早生中熟桑树新品种农桑12号、农桑14号。
The early sprouting and mid maturing new mulberry varieties Nongsang 12 and Nongsang 14 were bred through hybridization, single plant selection, pedigree selection and regional test.
该试验用幼苗形态鉴定法对杂交水稻品种进行鉴定。
This experiment was conducted to identify hybrid rice cultivar with seedling morphology i-dentification method.
应用酸性聚丙烯酰胺凝胶电泳（A-PAGE）和基因组原位杂交（GISH）鉴定DH群体中的1RS/1BL易位系。
Acidic polyacrylamide-gel electrophoresis (A-PAGE) of gliadin and genomic DNA in situ hybridization (GISH)were employed to discriminate 1RS/1BL translocation DH lines from the normal 1B DH lines.
本文还针对RAPD技术在西瓜杂交种纯度鉴定上实际应用的可能性进行了讨论。
The possibility of application of RAPD technique on identification of watermelon hybrid purity was discussed.
利用海陆杂交技术所选育的长绒棉抗病材料在枯萎病病圃、重病田等抗病性鉴定，都表现很好的抗病性。
The resistance to Fusarium wilt of long-fibre cotton selected from sea-land hybrid was appraised by using sea-land hybrid techniques.
目的：建立抗麻痹性贝毒素GTX2，3单克隆抗体杂交瘤细胞系，制备抗GTX2，3单克隆抗体，并鉴定其免疫学特性。
Objective: To obtain hybridoma cell line which can secrete anti-gonyautoxin2,3 (GTX2,3) monoclonal antibodies(mAb) and analysis the character of the mAb.
目的构建DLX4酵母双杂交系统并鉴定其自激活作用。
Objective To construct a yeast-two hybrid system of DLX4 and identify its self-activation.
结合玉米ssr分子标记检测技术和单籽粒dna快速提取方法，DNA指纹数据库和判别标准可有效地用于玉米杂交种的亲子鉴定。
As SSR marker technique and DNA rapid extraction method established, the DNA fingerprinting database could effectively be employed to identify parentage relations among hybrids.
方法应用淋巴细胞杂交瘤技术，制备单克隆抗体，并对其进行鉴定。
Methods McAb were prepared and identified by applying the lymphocyte hybridoma technique.
因此，这些结果显示SSR分子标记技术可以很好地用于杂交玉米种子的纯度鉴定，即使是这个杂交种是来自两个非常接近的自交系的。
These results clearly demonstrate that SSR marker should be useful for assessing purity of maize hybrid, even if the hybrid derived from two related parental lines.
目的：鉴定用于肿瘤比较基因组杂交(CGH)微阵列细菌人工染色体(BAC)克隆的质量。
Objective To evaluate the quality of BAC clones for CGH microarrays in the detection of tumors.
并以玉米杂交种农大108的纯度鉴定为例，探索了该体系在玉米杂交种鉴定中实际应用的可行性。
The hybrid purity identification of Nongda108was used as an example to explore the possibility of application of SSR technique on maize hybrid identification.
结果：32例阳性标本经过聚合酶链杂交结果与常规鉴定结果相符。结论：聚合酶链反应杂交梳法鉴定结核杆菌简便、快速、特异性强，有较高的临床应用推广价值。
Results The detective results of 32 positive samples with PCR hybridization combing is a quick, simple and highly specific method in the detection of TB, and may has some values in clinical diagnosis.
1990年，采用病区病圃剪叶接种法，对杂交稻三系资源及组合3098份进行了鉴定。
In 1986-1990 the leaf inoculation method was used in a diseased area or nursery to identify 3098 three line materials and rice hybrids.
利用微卫星(SSR)分子标记对北方杂交粳稻骨干亲本进行了遗传差异鉴定和籼性程度分析。
Genetic differentiation and indica component degree analysis were conducted on the main parents of japonica hybrid rice in the northern China by simple sequence repeats (SSR).
以吉林省玉米主推杂交种21个组合为试材，利用RAPD法与盐溶蛋白法（PAGE）进行了鉴定品种与杂交种纯度的对比试验。
RAPD and salt-soluble protein PAGE analysis were used in seed purity assessment of 21 hybrid corn combinations in Jilin Province.
本研究所获得的结果对于新育成的超级杂交稻组合及其纯度的鉴定，以及为知识产权的保护可以提供具有司法效力的技术支持。
It is suggested that the result obtained in this study could provide the legal evidence to protect plant proprietary rights of the new super hybrid rice combinations breeded recently.
膜反向斑点杂交技术鉴定分枝杆菌菌种的灵敏度为92.49%，特异度为100%。
The 16S rRNA PCR-membrane reverse dot blot hybridization technique showed that the sensitivity was 92.49%, and the specificity was 100%.
并对亚克隆后的阳性杂交瘤细胞株所制备的抗体的生物学功能进行了初步鉴定。
And we identified some biological functions of the antibodies produced by the positive subclones.
用MBXN作为母本分别与无香味的品种日本晴、9311配制杂交组合，用KOH法鉴定F_2代群体中单株叶片的香味，进行遗传分析。
The genetic analysis was carried out by crossing MBXN with non-fragrant Nipponbare and 9311, and checking the fragrance of leaves in F_2 generation by KOH methods.
用MBXN作为母本分别与无香味的品种日本晴、9311配制杂交组合，用KOH法鉴定F_2代群体中单株叶片的香味，进行遗传分析。
The genetic analysis was carried out by crossing MBXN with non-fragrant Nipponbare and 9311, and checking the fragrance of leaves in F_2 generation by KOH methods.
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