该杂交瘤细胞系表达小鼠TH 。
筛选共得到一株阳性杂交瘤细胞，命名为4d11。
是研究筛选抗ibd V单抗杂交瘤细胞的可靠方法。
This is a reliable method to select the hybridoma cells for McAb of IBDV.
目的建立分泌抗华支睾吸虫代谢抗原的单克隆抗体杂交瘤细胞株。
Then the hybridoma cell lines secreting high titer of McAbs were screened.
对所得阳性杂交瘤细胞作染色体分析并对单克隆抗体进行特性分析。
The chromosome of the positive hybridoma and the character of the mAb were analysed.
对杂交瘤细胞株的染色体计数的结果发现染色体均数为90～98条。
The number of chromosome of the hybirdoma cell line was 90 to 98.
还揭示表达所述免疫球蛋白分子和单克隆抗体的杂交瘤细胞或其他细胞系。
Hybridomas or other cell lines expressing such immunoglobulin molecules and monoclonal antibodies are also disclosed.
并对亚克隆后的阳性杂交瘤细胞株所制备的抗体的生物学功能进行了初步鉴定。
And we identified some biological functions of the antibodies produced by the positive subclones.
方法利用B细胞杂交瘤技术，建立分泌抗总黄曲霉毒素单克隆抗体杂交瘤细胞株。
Methods Hybridoma cell line excreting monoclonal antibody against total aflatoxins was produced by using B cell hybridoma technique.
结果：该杂交瘤细胞株分泌抗人脑胶质瘤细胞单克隆抗体H12具有与脑胶质瘤细胞特异性结合的特性。
Result:The monoclonal antibody H12 against human glioma which the hybridizing cell secretions can consociate specifically with human glioma.
研究了电场强度、脉冲宽度、脉冲次数等电场参数对基因转化频率的影响和小鼠杂交瘤细胞最适电转移条件。
Electrical parameters affecting gene transfer, such as the field strength ( Voltage ), pulse durations and pulse frequency ect. and optimum transfer conditions in murine hybridoma cells were studied.
方法利用RT -PCR方法从杂交瘤细胞株2f 3中扩增出单克隆抗体重链可变区和轻链可变区基因。
Methods the genes were amplified from the heavy (VH) and light (VL) chain variable regions of hybridoma cell strain 2f3 by RT-PCR and identified by DNA sequencing.
方法用RT - PCR方法从能分泌特异性抗人afp单克隆抗体的杂交瘤细胞中分离纯化抗体VH和VL基因。
Methods VH and VL genes of anti-human AFP monoclonal antibody were cloned by RT-PCR from hybridoma.
目的：建立抗麻痹性贝毒素GTX2，3单克隆抗体杂交瘤细胞系，制备抗GTX2，3单克隆抗体，并鉴定其免疫学特性。
Objective: To obtain hybridoma cell line which can secrete anti-gonyautoxin2,3 (GTX2,3) monoclonal antibodies(mAb) and analysis the character of the mAb.
但人-人B细胞杂交瘤可吸收掉上述BCGF活性上清中的促杂交瘤细胞生长因子(HGF)活性，而对BCGF活性无明显影响。
Absorption of human-human hybridoma cells can abolish the human hybridoma growth factor (HGF) activity but not the BCGF activity.
结论：杂交瘤细胞株5e10能稳定的分泌目的单克隆抗体，利用该抗体初步研制的AFB_1免疫亲和柱的性能仍需进一步优化。
Conclusion: the cell line 5e10 could secret the purpose McAb, AFB_1 immunoaffinity column prepared by the McAb needs to be further optimized.
作者采用小鼠骨髓瘤细胞系与破伤风类毒素免疫的人外周血淋巴细胞进行融合，对影响人-鼠杂交瘤细胞制备人单抗成功的因素进行了探讨。
The cells from mouse myeloma cell line SP2/0 were fused with human peripheral blood lymphocytes from the donors primed with a conventional dosage of tetanus toxoid (TT).
方法应用淋巴细胞杂交瘤技术，制备单克隆抗体，并对其进行鉴定。
Methods McAb were prepared and identified by applying the lymphocyte hybridoma technique.
建立杂交瘤单抗亲和层析纯化抗原、抗原体外致敏淋巴细胞和RT-PCR克隆人抗体基因及噬菌体呈现技术构建人源抗体库的策略。
Strategy was established for construction of repertoire antibody library with affinity chromatography purifying antigen, antigen immunizing human lymphocytes, RT-PCR and phage display technology.
在T细胞杂交瘤中，转移到周围淋巴结的肿瘤细胞通常都有强的L-选择蛋白的表达。
T cells' hybrid tumor cells which specifically metastasize to lymph node highly express L-selectin.
目的探讨利用杂交瘤技术制备肿瘤疫苗的方法，研究肺癌树突状融合细胞的生物学特征。
Objective To explore the established method of tumor vaccine generated by hybrid technique and study the biological characteristics of lung cancer-dendritic fusion cell.
目的探讨利用杂交瘤技术制备肿瘤疫苗的方法，研究肺癌树突状融合细胞的生物学特征。
Objective To explore the established method of tumor vaccine generated by hybrid technique and study the biological characteristics of lung cancer-dendritic fusion cell.
应用推荐