采用较低的细胞密度铺板，加入含最小致死量G418的DMEM培养基进行再次筛选；
The cells were planked with lower cell density, and further screened in DMEM medium containing G418 MFD;
同时选用正常的NIH3T3细胞为对照，使用含有最小致死量g418及未加g 418的DMEM培养基进行培养，采用光学显微镜对G418抗性NIH3T3细胞进行观察。
Meanwhile, normal NIH3T3 cells were taken as controls, and cultured by G418 contain MFD and by DMEM medium without G418 respectively. Light microscope was used to observe G418R NIH3T3 cells.
同时选用正常的NIH3T3细胞为对照，使用含有最小致死量g418及未加g 418的DMEM培养基进行培养，采用光学显微镜对G418抗性NIH3T3细胞进行观察。
Meanwhile, normal NIH3T3 cells were taken as controls, and cultured by G418 contain MFD and by DMEM medium without G418 respectively. Light microscope was used to observe G418R NIH3T3 cells.
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