本论文是上述微流体数字化技术在细胞显微注射技术上的实验研究。
In this paper, the applied research of cell microinjection is based on the micro-fluidic digital technology above.
经显微注射后的受精卵适当培养,选择优质胚胎进行受体小鼠的输卵管移植;
The donor and acceptor mice were treated to oestrus synchronization or superovulation at the same time and the fertilized ova were collected for microinjection.
根据三次显微注射的结果,注射菌液后的赤眼蜂的成活率平均值仅为2.1%。
According to the results of three generations of microinjection, the average of survival rate is only to 2.1%.
该技术主要包括:目的基因和靶细胞的制备、样品的灌注以及对细胞的显微注射。
The main procedures were: to prepare the target genes and cells, to fill the sample into the glass capillary injector and to proceed microinjection.
回顾了目前利用微机电(MEMS)技术在高效并行显微注射和胚胎定位方面的工作;
In this paper, we reviewed our work on high -throughput microinjection and positioning of embryos using the MEMS technologies.
选用较低的基因浓度,良好的操作介质CZB,使用质量好的受精卵,会提高显微注射的效率。
Lower concentration of the microinjected DNA, choosing CZB and zygotes with good quality can enhance efficiency of microinjection.
研制了硅微光学力传感器,并用来研究和优化显微注射器自组装定位芯片的几何结构和操作程序。
Silicon micro -optical force sensors were also developed to study and optimize the geometry and operation procedure of the microinjectors and self-assembly positioning chip.
本发明公开了一种基于显微注射把纳米材料放入活体细胞中的方法,属于生物细胞学中的细胞操作技术。
The invention discloses a microinjection-based method for implanting nanometer material in a living cell, belonging to the cell manipulation technology of biological cytology.
向花柱引导组织中显微注射纯化钙调素可促进花粉管束伸长,而注射钙调素抗血清可抑制花粉管束伸长;
Pollen tube growth in style transmitting tissue was accelerated when the style was microinjected with exogenous purified CaM(Fig. 5), but was inhibited by microinjection with anti-CaM serum (Fig. 6 ).
用显微注射法将胡子鲶总DNA导入江团受精卵中。孵育后对转化和对照鱼的外形、适应性进行比较分析;
The morpha and adaptabilities of transformed Leiocassis longirostris Gunther (LLG) which induced the total DNA from Coarias. sp by microinjection were compared to its control.
转基因是近年来在生命科学领域广泛应用的新兴技术,目前80%以上的转基因动物模型是利用显微注射法构建完成的。
Gene transfer is a new and widely used technology in bioscience field. At present over 80 percent of transgenic animal models are constructed by means of microinjection.
目前用于油菜基因转化的方法有根癌农杆菌介导法、基因枪法、PEG法、激光微束穿刺法、显微注射法和花粉介导法等。
At present, there are several methods of transformation in rape, including tumefaciens-medidated genetic etc, particle gun, PEG untake, vialaser microbeam puncture and microinjection.
采用显微注射系统对SHG-44细胞进行单细胞微量注射技术的探讨,并观察去甲二氢愈创木酸(NDGA)注射后的作用。
A new microinjection system was used to investigate the technology of single cell microinjection in SHG-44 cell line and the effect of differentiation induced by nordihy droguaiaretic acid(NDGA).
通过以上研究为应用显微注射技术人工诱导赤眼蜂的产雌孤雌生殖,提高赤眼蜂的生防效能提供了理论依据并奠定了实验基础。
The conclusion drawn in this research will make a preparation of experiment technique and academic evidence for further study and application of Wolbachia in Trichogramma in the field of bio-control.
方法利用有机玻璃、注射器、压力表等制作一种用于显微手术教学培训的眼球固定台。
With the organic glass, injector, pressure gauge and so on, we make one kind of eyeball fixed platform easy and flexible to operate.
本文报道用血管注射、显微摄影及扫描电镜等方法,研究了42侧婴幼儿胫骨的骨髓血液供应。
The blood supply of the tibial bone marrow in infants was studied in 42 fresh lower limbs by injection with chinese ink and observed with microangiography and SEM methods.
如果新的显微针技术成功了的话,我们将很快享受甚至你都不能意识到的药物注射。
If new microneedle technology is successful, we may soon have needles that inject medicine without your even knowing it.
这篇论文主要研究了用粉末注射成形工艺制造各种高性能与功能烧结材料时,需要的显微组织控制。
This paper focused on the microstructural controls needed for various high performance and functional sintered materials through powder injection molding process.
在注射3,24,72,120小时后,分别用光学显微镜、荧光显微镜、共聚焦显微镜观察实验结果。
Observation was done at 3, 24, 72, and 120 hours after injection using light microscopy, fluorescent microscopy, and confocal microscopy.
应用酶组织化学、透射电子显微镜及淋巴管墨汁活体注射法对大鼠小肠系膜淋巴管的发育进行了研究。
The embryonic development of the lymphatics in mesentery of small intestine in Wistar rats is studied By using the methods of enzyme histochemistry, TEM and injection of special ink.
方法用显微计数法分别测定清开灵注射液与输液配伍前后药液的不溶性微粒数。
Methods by using microscope counter the amount of insoluble particles in Qingkailing injections and the liquid mixed by Qingkailing injections and transfusion fluids.
方法用显微计数法分别测定清开灵注射液与输液配伍前后药液的不溶性微粒数。
Methods by using microscope counter the amount of insoluble particles in Qingkailing injections and the liquid mixed by Qingkailing injections and transfusion fluids.
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