目的建立昆虫杆状病毒表达系统表达人II型CD 74胞外端片段的技术平台。
Objective to establish an expression system of human II CD74 extracellular fragment by insect baculovirus vector expression system in insect cells.
间接免疫荧光、ELISA和免疫印迹检测证明，含SO.7片段的三种重组杆状病毒均在昆虫细胞中表达出相应的活性蛋白。
By IFA, ELISA and Western blot experiments, it was proved all the recombinant baculoviruses containing SO. 7 segment could express biologically active proteins in insect cells.
该病毒作为载体，应用在杆状病毒-昆虫真核生物表达系统。
It is applied as a carrier in expression system of granulosis virus insect eucaryotic biology.
然后使用重组杆状病毒技术使这些S1亚单位在昆虫细胞和昆虫幼虫中实现了高水平表达。
The modified S1 subunits were abundantly expressed by recombinant autographa californica nuclear polyhedrosis viruses in insect cells and larva.
结论：杆状病毒载体能够介导HHV - 8肿瘤转化基因k12编码的蛋白在昆虫细胞中表达。
Conclusion: Baculovirus transfer vector may mediate the coding protein of HHV-8 K12 gene expressed in insect cells.
系统分析表明，杆状病毒IAP基因可能是病毒与鳞翅目昆虫在长期的进化过程中从宿主基因组中获得的。
Phylogenetic analysis of IAP genes and lots of evidence sppport the origin of this viral gene by capture of a host gene early in the evolution of Lepidoptera.
近年来杆状病毒介导的昆虫表达系统得到了广泛地应用，本课题通过该系统表达CYP2B6重组酶，并进行活性鉴定。
In this study, CYP2B6 recombinant enzyme was obtained using baculovirus expression system and its catalytic activity was measured using bupropion as probe.
近年来杆状病毒介导的昆虫表达系统得到了广泛地应用，本课题通过该系统表达CYP2B6重组酶，并进行活性鉴定。
In this study, CYP2B6 recombinant enzyme was obtained using baculovirus expression system and its catalytic activity was measured using bupropion as probe.
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