拓扑异构酶II基因突变；
然后拓扑异构酶i将P CR产物按照正确的方向连接。
Topoisomerase I then ligates the PCR product in the correct orientation.
DNA拓扑异构酶与断开dna链和改变其拓扑结构有关。
DNA strand scission and topology changes are catalyzed by DNA topoisomerases.
还对分化诱导剂、拓扑异构酶抑制剂、抗微管药和生物反应调节剂的筛选方法进行了评述。
In addition, the paper reviewed screening methods for differentiation inducers, DNA topoisomerase inhibitors, antimicrotubule drugs and biological response modifiers.
目的：研究紫草素对DNA拓扑异构酶i催化活性和K5 6 2白血病细胞凋亡的影响。
AIM: to study the effects of shikonin on the catalytic activity of DNA topoisomerase I and apoptosis of K562 leukemia cells.
另一个例证是，葛兰素史克公司的研究人员最近描述了一种以iia型拓扑异构酶为作用目标的新的抗菌剂。
Another example is that of researchers at GlaxoSmithKline who recently described a novel class of antibacterial agents that target type IIA topoisomerases.
目的：探讨DNA拓扑异构酶在卵巢浆液性、黏液性囊腺癌中的表达及与细胞增殖、凋亡的关系。
Purpose to investigate the relationship between DNA topoisomerase and cell proliferation, apoptosis in ovarian carcinomas.
结论：小鼠tfar19是与人TFAR19高度同源的新基因，与DNA拓扑异构酶i可能有功能相关性。
Conclusion: The mouse TFAR19 sequence is highly homologous with human TFAR19 and may have functional relationship with DNA topoisomerase I.
结论铜绿假单胞菌的耐药性日趋严重，两类拓扑异构酶基因突变和泵出机制形成了铜绿假单胞菌对喹诺铜类药物耐药的主要机制。
The resistance to quinolones in clinical isolates of Pseudomonas aeruginosa is due to the mutations of two types of topoisomerase plus efflux pump.
依托泊苷能稳定酶催化性循环中的一种共价的拓扑异构酶ii裂解的DNA中间复合物，导致基因组不稳定性和细胞死亡(2,3)。
Etoposide stabilizes a covalent topoisomerase II-cleaved DNA intermediate complex in the catalytic cycle of the enzyme, leading to genomic instability and cell death (2, 3).
依托泊苷能稳定酶催化性循环中的一种共价的拓扑异构酶ii裂解的DNA中间复合物，导致基因组不稳定性和细胞死亡(2,3)。
Etoposide stabilizes a covalent topoisomerase II-cleaved DNA intermediate complex in the catalytic cycle of the enzyme, leading to genomic instability and cell death (2, 3).
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