通过双荧光素酶报告基因测定法测定了IGFBP-3对由三碘甲状腺素刺激的生长激素启动子活性的影响。
The effect of IGFBP-3 on the growth hormone promoter activity stimulated by triiodothyronine was determined by dual-luciferase reporter assay.
方法采用荧光素酶报告基因方法。
我们用荧光素酶报告基因的方法，探讨了这个问题。
We addressed this question with the method of luciferase reporter gene.
对发现的突变，进行荧光素酶报告基因瞬时表达研究。
The reporter gene was used to study the mutation influencing the activity of promoter.
之后让两种蛋白都在具有一种报告基因的酵母细胞中表达。
Both proteins are then expressed in yeast cells that have a reporter gene.
GF P基因作为报告基因已被广泛应用于各种生物的研究中。
As a reporter gene, GFP has been used in a variety of biological researches.
随着化合物剂量的增加，报告基因的表达降低（P<0.05）。
The genetic expression decreased when dose of compound increased(P<0.05).
当前，报告基因显像的焦点是选择何种报告基因探针进行基因显像。
At present, the key problem is focused on the selection of what kind of reporter gene probe would be.
荧光素酶报告基因分析技术在药物的高通量筛选中起着重要的作用。
Luciferase reporter gene technique plays an important role in high throughput screening.
目的构建以绿色荧光蛋白（GFP）为报告基因的酿酒酵母表达载体。
Objective To construct saccharomyces cerevisiae expression vector with GFP as report gene.
我们使用了依赖HCVIRES表达萤火虫荧光素酶的报告基因质粒。
We used a reporter gene plasmid in which firefly luciferase expression is dependent on the HCV IRES.
本研究建立的AR报告基因试验可作为化学物雄激素活性筛选的理想方法。
The AR) reporter gene assay is useful in screening androgenic effect of chemicals.
结果构建并鉴定了系列截短的NOS1 启动子萤光素酶报告基因载体；
Results A series of truncated NOS1 promoter luciferase reporter vectors were constructed and identified.
利用这个报告基因为基础的药物筛选系统，筛选了100多种中药提取物。
Using this cell-based drug screening system, more than 100 herbal extracts were screened.
结果成功构建了以GFP为报告基因的酿酒酵母载体，并在酵母中得到表达。
Results Saccharomyces cerevisiae expression vector with GFP as report gene was constructed and expressed successfully.
基因转染后，GH 3细胞特异性表达报告基因，并被治疗基因特异性杀伤。
After gene transfection, GH3 cell specifically expressed reporter gene and killed specifically by therapy gene.
目的：构建缺氧诱导表达载体，以介导报告基因在缺氧环境下的特异、高效表达。
Objective: to construct hypoxia-inducible expression vector, which mediated reporter gene to express specially and efficiently in hypoxia environment.
为了阐明调控机制，我们采用了点突变实验和在CHO细胞系中的报告基因分析。
To clarify the regulatory mechanism, site-directed mutagenesis and reporter gene assay in the CHO cell line were used.
建立以氯霉素乙酰化酶(CAT)为报告基因的雄激素受体(AR)报告基因试验。
To develop an androgen receptor (AR) reporter gene assay in which the reporter gene is chloramphenicol acetyltransferase (CAT).
肝脏疾病的分子影像学研究，注重于报告基因、质粒构建和纳米单晶磁性粒子的研究。
The main task for post-doctorate is molecular imaging research for hepatic diseases, especially in the fields of report gene, plasmid construction, and monocrystalline iron oxide nanocompound (MION).
瞬时转染人胚胎肾细胞293t，用荧光素酶报告基因检测这两种剪切形式的转录活性。
The transcriptional activity of the two splicing variants was detected with a luciferase reporter in transient transfections of human embryonic kidney293T cells.
创伤后并发器官损害病人的荧光素酶报告基因活性显著高于无器官损害者，其死亡率也较高。
On the other hand, the activity of LUC was higher in the organ damagd patients than that in the non-organ damagd patients, with a high mortality rate.
目的研究含尤文家族肿瘤ews FLI1结合序列的DTA载体对报告基因表达的抑制作用。
Objective to study the DTA vector containing the Ewing family of tumors EWS-FLI1 binding sequence depresses the co-transferred luciferase reporter plasmid.
结论采用绿色荧光蛋白为报告基因的真核细胞转染技术中，脂质体法是效率高、安全性大的方法。
ConclusionUsing GFP as a report gene in eukaryotic cell transfection, the lipofectamine method could get a higher transfection efficiency and higher survival rate for cells.
目的构建磷脂酰肌醇蛋白聚糖3 (GPC3)启动子荧光素酶报告基因载体，并验证其转录活性。
ObjectiveTo construct glypican-3 (GPC3) promoter luciferase reporter gene vector, and to analyze its transcriptional activity.
本文主要综述了受体基因作为报告基因与治疗基因共同转基因并通过放射性核素显像监测表达的方法。
In the paper we review several strategies to monitor the gene therapeutic efficacy by certain receptor gene as reporter gene transferred together with therapeutic ge...
携带GF P报告基因的慢病毒可感染成熟脂肪细胞，感染率约为80%，且细胞被感染后状态良好。
Lentivirus with GFP reporter can infect differentiated adipocytes. The infection efficiency was about 80%, and infected cells were in good conditions.
该启动子的成功克隆和其报告基因载体的构建，为研究SOD2的基因表达调控机制提供了重要基础和工具。
The reporter gene vector driven by SOD2 promoter will provide an experimental tool for the further study on the regulatory mechanism of the SOD2 expression.
将其与GUS报告基因融合在一起，构建了植物表达载体，并由农杆菌介导法导入水稻品种‘中花11’中。
It was fused with GUS reporter gene to construct a plant expression vector and the vector was transformed into Zhonghua 11, a rice variety, by Agrobacterium meditation.
方法：通过PCR的方法克隆GCLC基因的部分转录调控区基因，构建表达虫荧光素酶报告基因的质粒；
METHODS: The regulating region of the human GCLC gene was cloned by PCR method to construct the wild type plasmid, which expressed luciferase reporting gene.
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