另外获得了‘萨蔓莎’和‘金斯莱克’的抗性愈伤组织。
The Kan-resistant callus of 'Samantha' and 'Jimthyianky' are acquired.
以CC培养基作为共培养后抗性愈伤筛选的基本培养基有利于抗性愈伤组织的筛选培养。
The CC medium was an efficient selectable medium for screening hygromycin resistant calli after co cultivation.
最佳处理条件下转化683枚八棱海棠叶片，共得到138个抗性愈伤组织和15株抗性苗，转化率为2.2%。
Total 683 leaves were transferred under the optimum treatment condition, then 138 resistant callus and 15 resistant shoots were obtained (transformation rate 2.2%).
处理后的愈伤组织接种到含有15 %聚乙二醇（PEG）的筛选培养基上，获得对水分胁迫具有抗性的愈伤组织，诱变率为5 .4 %。
When the treated callus inoculated on the selection medium contains 15% PEG, 5.4% of water stress resistant mutants were induced.
抗性植株和愈伤组织的分子鉴定正在进行中。
Our further molecular assays on resistant plants and calli are also being conducted.
对于继代时间较长的愈伤组织，高渗处理可提高抗性愈伤的比率。
High osmotic treatment may increase the possibility of transformation, especially for calli of 6 months or older.
幼胚诱导的愈伤组织，经潮霉素抗性筛选，得到可育的再生植株。
The regenerated fertile plants were obtained by effective selection for hygromycin B resistance.
幼胚诱导的愈伤组织，经潮霉素抗性筛选，得到可育的再生植株。
The regenerated fertile plants were obtained by effective selection for hygromycin B resistance.
应用推荐