本课题基于可重复使用的免疫磁珠技术,抗原表位分析预测技术,多肽合成及筛选技术,建立了一种简捷快速高效的特异抗体检测方法。
The method is based on the the immunomagnetic beads that can be used repeatedly, the technology of investigating epitopes and the technology of synthesizing multiple antigen peptides.
本研究获得的单克隆抗体将为今后深入研究PRRSV病毒结构和功能,分析GP5蛋白的抗原表位,设计有效的新型疫苗等提供有益帮助。
The MAbs against PRRSV GP5 can be used for further analysis of the structure and function of PRRSV, identification of epitopes on GP5, which will be helpful for new vaccine design.
在线用蛋白质二级结构预测软件分析MAGE - 4抗原表位,PCR获取MAGE - 4抗原特征表位基因。
Analyze MAGE-4 antigen epitope by online predict software of protein secondary structure and amplify the epitope gene by PCR.
目的:分析患者血清中抗hcv抗体的抗原表位。
Objective: epitope analysis of anti-HCV antibodies isolated from patients serum.
通过噬菌体肽库技术分析抗体所识别的抗原表位。
The mAb epitope was also analyzed using peptide phage display technology.
方法:应用生物信息学分析软件分析SARS冠状病毒S蛋白的抗原表位序列,设计全新的抗原表位编码基因序列,构建表达载体并在大肠杆菌中表达该基因。
Methods: By analyzing the antigen epitope of SARS-CoV s protein with antigenic peptides prediction software, a new gene was designed, and its expression vector was constructed.
方法:应用生物信息学分析软件分析SARS冠状病毒S蛋白的抗原表位序列,设计全新的抗原表位编码基因序列,构建表达载体并在大肠杆菌中表达该基因。
Methods: By analyzing the antigen epitope of SARS-CoV s protein with antigenic peptides prediction software, a new gene was designed, and its expression vector was constructed.
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