建立杂交瘤单抗亲和层析纯化抗原、抗原体外致敏淋巴细胞和RT-PCR克隆人抗体基因及噬菌体呈现技术构建人源抗体库的策略。
Strategy was established for construction of repertoire antibody library with affinity chromatography purifying antigen, antigen immunizing human lymphocytes, RT-PCR and phage display technology.
再将RB肿瘤抗原致敏树突状细胞，与自体淋巴细胞共孵育后，能诱导出特异性杀伤肿瘤细胞的CTL。
The dendritic cells were induced by RB tumor antigen and then it cooperated with RB tumor lysates in vitro to induce antigen specific CTL which can kill the SO-RB50 target cells specifically.
再将RB肿瘤抗原致敏树突状细胞，与自体淋巴细胞共孵育后，能诱导出特异性杀伤肿瘤细胞的CTL。
The dendritic cells were induced by RB tumor antigen and then it cooperated with RB tumor lysates in vitro to induce antigen specific CTL which can kill the SO-RB50 target cells specifically.
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