这类B细胞表现出早熟和低抗hiv的能力下降，分泌抗体的能力下降。
B cells showed such precocious and low anti-HIV decline in the ability, the ability of antibody production decline.
方法利用B细胞杂交瘤技术，建立分泌抗总黄曲霉毒素单克隆抗体杂交瘤细胞株。
Methods Hybridoma cell line excreting monoclonal antibody against total aflatoxins was produced by using B cell hybridoma technique.
结果：该杂交瘤细胞株分泌抗人脑胶质瘤细胞单克隆抗体H12具有与脑胶质瘤细胞特异性结合的特性。
Result:The monoclonal antibody H12 against human glioma which the hybridizing cell secretions can consociate specifically with human glioma.
研究者希望能设计一种治疗性的疫苗，减缓HIV的疾病进程，使失效的B细胞恢复分泌高质量抗体的能力。
The researchers hope to design a therapeutic vaccine, slow HIV disease process, so that failure of B-cell secretion restore the ability of high-quality antibodies.
方法用RT - PCR方法从能分泌特异性抗人afp单克隆抗体的杂交瘤细胞中分离纯化抗体VH和VL基因。
Methods VH and VL genes of anti-human AFP monoclonal antibody were cloned by RT-PCR from hybridoma.
目的建立分泌抗华支睾吸虫代谢抗原的单克隆抗体杂交瘤细胞株。
Then the hybridoma cell lines secreting high titer of McAbs were screened.
结论：杂交瘤细胞株5e10能稳定的分泌目的单克隆抗体，利用该抗体初步研制的AFB_1免疫亲和柱的性能仍需进一步优化。
Conclusion: the cell line 5e10 could secret the purpose McAb, AFB_1 immunoaffinity column prepared by the McAb needs to be further optimized.
结论：杂交瘤细胞株5e10能稳定的分泌目的单克隆抗体，利用该抗体初步研制的AFB_1免疫亲和柱的性能仍需进一步优化。
Conclusion: the cell line 5e10 could secret the purpose McAb, AFB_1 immunoaffinity column prepared by the McAb needs to be further optimized.
应用推荐