结论P CR扩增法可以获得优良的DNA分子量标准。
使用一步法巢式逆转录聚合酶链扩增法(RT - PCR)。
One step nested reverse transcriptase-polymerase chain reaction (RT-PCR) was used.
用巢式PCR、引物延伸预扩增法扩增单细胞，扩增率分别为90％和85％。
Amplification rate of nest-PCR and PEP was 90% and 85%, respectively.
结论：体外扩增法成功地从兔骨髓中分离培养出具有血管内皮祖细胞特征的细胞群体。
CONCLUSION: Cell colony with the feature of EPCs can be isolated and cultured from rabbit bone marrow by in vitro amplification method successfully.
用端粒重复序列扩增法（TRAP）半定量方法测定细胞染毒前后端粒酶活性的变化；
The changes of telomerase activity after treatment was detected and quantified by telomeric repeat amplification protocol (TRAP) semi_quantitative analysis.
方法取19例颅内肿瘤和5例脑外伤患者脑脊液标本，应用改良的银染端粒重复序列扩增法(TRAP)进行端粒酶活性检测。
Methods a modified telomeric repeat amplification protocal (TRAP) by silver staining was performed in 19 cases of intracranial tumors and 5 cases of brain injuries.
方法采用端粒重复序列扩增法一酶联免疫（PCR-TRAP）检测70例肺癌手术患者癌组织中的端粒酶活性，并与70例肺部良性疾病患者对照组的检测结果进行比较。
Methods Use PCR-TRAP detection of 70 cases of lung cancer patients' telomerase activity in cancer tissue. and 70 patients with benign lung disease and the results were compared.
与扩增曲线观察法结果一致。
目的探讨本地区性传播疾病（STD）患者沙眼衣原体（CT）、解脲支原体（UU）的感染现状及使用核酸扩增杂交梳法检测CT、UU的价值。
Objective To detect the status of CT and UU infection in local (STD)patients, and the value of measuring CT and UU with Nucleic Acid Amplification Hybridization Climb method.
以随机扩增DNA多态性法对16种支孢26株菌的DNA指纹图谱作了分析。
Random amplification of polymorphic DNA(RAPD)assay was used to study the finger-printing of 26strains from 16specie s.
结果运用群特异多重PCR法可特异性的扩增出当前流行群副溶血性弧菌的目的基因片段，可大大缩短鉴定时间。
Results The aim gene fragment can be amplified by a multiplex PCR assay, it largely reduce the time of identification.
采用聚合酶链反应(PCR)方法扩增rho基因第1 ~ 5外显子和第1内含子基因片段，用直接dna测序法筛查rho基因突变。
Extron 1-5 of RHO gene was amplified by polymerase chain reaction (PCR), and the mutation of RHO gene was screened by direct DNA sequence measurement.
用双脱氧链末端终止法测序结果表明，NS 1基因扩增片段5端的部分碱基序列并未发生改变。
The result of the nucleotide sequence determined by dideoxy chain-termination method indicated that 5 '-terminal nucleotide sequence of the amplified NS1 fragment was not changed.
方法分离、鉴定并扩增取自10 ~ 14周药物流产胎儿大脑皮质的神经干细胞，应用pcr -ELISA法检测神经干细胞端粒酶活性。
Methods the neural stem cells were isolated, identified and amplified from the cortex of aborted human embryo with 10 to 14-week gestational age, then telomerase activity was detected by PCR-ELISA.
遗憾的是，亚甲蓝扩增染色内镜法仅探及3个经组织学证实的非典型增生患者中的1个。
Unfortunately, methylene blue magnification chromoendoscopy detected only 1 of 3 patients with biopsy-documented dysplasia.
方法整合子PCR法扩增整合子的可变区；
Methods The variable region of integron was amplified by integron PCR.
利用改良的SDS法提取DNA效果很好，RAPD反应时，DNA模板中RNA对扩增结果无影响，SDS、氯仿、异丙醇等小分子物质对扩增结果有干扰作用。
The modified SDS method which was used to extract DNA got good results. During RAPD , RNA in the DNA template had no effects on the amplification results.
方法建立随机扩增多态性DNA聚丙酰胺凝胶电泳（RAPD-PAGE）指纹分型法；
Methods To establish a fingerprinting method by random amplified polymorphic DNA and polyacrylamide gel electrophoresis (RAPD-PAGE).
用双脱氧链末端终止法测序结果表明，NS1基因扩增片段5’端的部分碱基序列并未发生改变。
The result of the nucleotide sequence determined by dideoxy chain-termination method indicated that 5'-terminal nucleotide sequence of the amplified NS1 fragment was not …
目的探讨改良扩增前引物延伸(ipep)法对痕量DNA样本s TR检测分型的效果。
Objective To study the effect of modified improved primer extension preamplification (IPEP) on STR analysis of trace DNA.
方法：PCR循环测序法是将PCR扩增与核酸序列分析相结合的一种研究方法。
Methods: PCRbased cycle DNA sequencing is a method that combines PCR amplification with DNA sequencing.
方法采用聚合酶链反应扩增该家系患者和健康对照个体atp2c1基因的全部外显子，直接测序法进行DNA测序，100例无亲缘关系的正常人作为对照。
Method all exons of ATP2C1 gene were analyzed with polymerase chain reaction and DNA sequencing in all patients of this family and 100 unrelated population-match controls.
方法采用端粒重复序列扩增文件-酶标法（TRAP-ELISA）测定尖锐湿疣皮损中端粒酶的活性，并与正常皮肤和恶性肿瘤对照比较。
Methods The telomerase activity was detected by TRAP( telomeric repeat amplification protocol) -ELISA which based upon PCR amplification of the initial telomerase product and detected by ELISA.
多重PCR法扩增待杂交并于芯片上微型测序的靶DNA。
The DNA reference targets for hybridization and subsequent on chip minisequencing was generated by multiplex PCR.
方法设计特定引物，用PCR方法对DC-SIGNR基因绞链区重复序列进行扩增，用凝胶电泳法对其产物进行分析。
Methods Specific primers for amplifying DC-SIGNR repeat regions were designed, and the PCR products were analyzed by electrophoresis.
结果：倒置显微镜观察，悬浮细胞培养法A组和组织块培养法B组扩增的角膜缘上皮细胞均能在羊膜上单层生长。
Results: A monolayer of cultured corneal limbal epithelial cells was observed by inverted microscope in both A group and B group.
说明改良的CTAB法能成功用于梨属植物DNA提取，建立的最佳反应体系可用于梨属植物RAPD扩增。
It explained that reformed CTAB method could extract the genomic DNA of Pyrus successfully, and the optimum RAPD reaction system could amplificate RAPD in Pyrus L…
用核酸扩增荧光定量法检测血清、胃黏膜HBVDNA ，综合分析各检测值对肝胃不和证积分的意义。
Liver function and the markers of HBV were detected. The contents of HBV- DNA in serum and in gastric mucosa were assayed respectively by fluorescence quantitative polymerase chain reaction (FQ-PCR).
用核酸扩增荧光定量法检测血清、胃黏膜HBVDNA ，综合分析各检测值对肝胃不和证积分的意义。
Liver function and the markers of HBV were detected. The contents of HBV- DNA in serum and in gastric mucosa were assayed respectively by fluorescence quantitative polymerase chain reaction (FQ-PCR).
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