方法从胚胎大鼠的大脑皮质、海马、纹状体等组织分离神经干细胞，用无血清培养技术在体外进行培养、扩增、传代和诱导分化。
The advantage of serum free and clone culturing technology was performed to isolate, culture, passage and induce neural stem cells from embryonic rat cortex, hippocampus and striatum.
另外研究血管内皮生长因子(VEGF)和传代培养对细胞分化、扩增动力学和细胞凋亡的影响。
In addition, we tested the effect of vessel endothelial growth factor (VEGF) and passage on cell differentiation, expansion kinetics and apoptosis.
结果原代的小鼠培养骨髓基质细胞能很好的维持精原干细胞的非分化性扩增。
The feeder layers of bone marrow stromal cells allowed spermatogonial stem cells to proliferate well and remain undifferentiated.
对扩增的细胞进行免疫表型、定向分化、造血祖细胞集落分析及脾集落形成单位的研究。
The expanded cells were further analyzed by phenotype, committed differentiation, progenitor colony assay as well as colony forming unite spleen.
目的：探讨骨髓间充质干细胞体外扩增及其向内皮样细胞定向分化条件。
AIM: to explore the conditions of isolation, expansion and differentiation of bone marrow mesenchymal stem cells (MSCs) into endothelium like cells in vitro.
方法：利用无血清培养和细胞克隆培养技术，从胚胎大鼠海马、纹状体、脊髓等区分离神经干细胞，进行体外扩增培养、传代、贴壁分化观察。
Methods: The advantage of serum free and clone culturing technology was performed to isolate, culture and passage neural stem cells from embryonic rat hippocampus, striatum and spinal cord.
目的：了解造血生长因子的不同组合对人脐血CD34+细胞的扩增效应及分化特性。
Objective: To explore the role of human hematopoietic growth factors (HGFs) in proliferation and differentiation of cord blood CD34 cells.
结论：agm基质细胞在有效扩增小鼠hsc同时，能很好地维持HSC自我更新及多向分化的潜能。
CONCLUSION: AGM-derived stromal cells significantly support the expansion of HSC, and also maintain the self-renewal activity and multi-lineage differentiation of HSC in vitro.
背景：骨髓间充质干细胞取材方便，易于体外扩增，其体外培养方法及诱导分化仍需要改良。
BACKGROUND: Bone marrow mesenchymal stem cells (BMSCs) are in advantages of easy collection and amplification in vitro, but the culture and induction in vitro still need to be modified.
目的：系统的研究小鼠胚胎干细胞的提取、体外培养扩增和向成骨细胞定向诱导分化。
Objective: research systematically into the isolation, culture and direct differentiation to osteoblast of embryonic stem cells.
对扩增细胞进行再培养后再进行分化。
After reculturing, these expanded cells can then be driven to differentiation.
【译】芳香烃受体造血祖细胞的扩增和分化的指示。
The aryl hydrocarbon receptor directs hematopoietic progenitor cell expansion and differentiation.
目的：探讨人脂肪源干细胞在体外分离培养扩增的方法及向成骨细胞诱导分化的能力。
OBJECTIVE: to establish a method for the isolation and culture of human ADSCs and to explore the ability of osteogenic differentiation.
用低分化鼻咽癌细胞株CNE-2 DNA转染NIH3T3细胞，获得的转化细胞株经扩增进行二轮和三轮转染。
We have obtained foci of transformed NIH3T3 cells through transfection of human DNA from CNE-2 (an undifferentiated epithelial cell line of nasopharyngeal carcinoma).
目的探讨利用肝损伤早期和淤胆血清培养体系从大鼠骨髓细胞中筛选、诱导分化和扩增肝干细胞亚群，以快速、高效地获取骨髓源性肝干细胞。
More and more reports about the application of liver stem cells in hepatic diseases appeared, especially about bone marrow-derived liver stem cells isolated from bone marrow.
方法：取健康新西兰幼兔骨髓，密度梯度离心法分离骨髓间充质干细胞并进行体外扩增培养、成骨诱导分化及鉴定。
METHODS: Bone marrow was obtained from healthy New Zealand rabbits, by using density gradient centrifugation BMSCs were isolated and were then cultured in vitro.
方法：取健康新西兰幼兔骨髓，密度梯度离心法分离骨髓间充质干细胞并进行体外扩增培养、成骨诱导分化及鉴定。
METHODS: Bone marrow was obtained from healthy New Zealand rabbits, by using density gradient centrifugation BMSCs were isolated and were then cultured in vitro.
应用推荐