从人胚胎生殖嵴、肠系膜中消化分离的原始生殖细胞，将其接种在人子宫内膜成纤维细胞饲养层上传代培养。
PGC from genital ridge and mesenterium of human embryo was incubated on fibroblast feeder layers for subculture.
观察鼠囊胚在小鼠原代胎儿成纤维细胞饲养层上的生长行为。
To observe the mouse blastocyst growth behavior on primary mouse embryonic fibroblast feeder layers (PMEF).
人胚胎成纤维细胞饲养层在ES细胞分离过程中具有较好的作用。
Human embryonic fibroblast feeder played a positive role during the ES cells isolation procedure.
目的探讨建立有效的小鼠胚胎成纤维细胞饲养层，用于胚胎干细胞的培养。
Objective To study the isolation and culture and application of Kunming mouse embryonic fibroblasts(MEF).
目的：建立ICR小鼠胚胎成纤维细胞饲养层培养体系，用于胚胎干细胞建系及研究。
Objective: To establish feeder layer culture system of ICR mouse embryonic fibroblast (MEF) cells for isolation and culturing of embryonic stem (es) cells.
结果分别获得了传16代的胎鼠成纤维细胞和传7代的人胎儿成纤维细胞，建立了人胚胎干细胞饲养层的培养体系。
Results Acquired 16 passages in mouse and 7 passages in human and established the culture system of human embryonic stem-like cells.
结果分别获得了传16代的胎鼠成纤维细胞和传7代的人胎儿成纤维细胞，建立了人胚胎干细胞饲养层的培养体系。
Results Acquired 16 passages in mouse and 7 passages in human and established the culture system of human embryonic stem-like cells.
应用推荐