目的尝试利用腺病毒介导慢病毒载体的细胞感染以提高转染效率。
Purpose To test the hypothesis that adenovirus may improve gene transfer efficiency of lentiviral vector by mediating viral entry.
目的：探讨慢病毒载体在白血病细胞中的基因转导效率，为白血病基因治疗提供关键依据。
Objective to investigate the gene transduction efficiency of lentiviral vector in leukemia cells to provide key basis for leukemia gene therapy.
目的：探讨慢病毒载体在白血病细胞中的基因转导效率，为白血病基因治疗提供关键依据。
Abstract: Objective to investigate the gene transduction efficiency of lentiviral vector in leukemia cells to provide key basis for leukemia gene therapy.
结论：基于慢病毒载体基因转导的高效性，该载体系统可作为白血病细胞基因转导的极好工具。
Conclusion This lentiviral vector is an excellent gene transduction system for leukemia cells because of its high gene transduction efficiency.
本发明公开了一种表达胶质源性神经营养因子的慢病毒载体及其用途，属于医学生物技术领域。
The invention discloses a lentiviral vector for expressing the glial cell line-derived neurotrophic factor and applications thereof, which belong to the field of medical biotechnology.
构建成功的新型慢病毒载体为今后的基因治疗奠定了基础，也为慢病毒滴度的确定提供了一种新的可能。
This lays the foundation for the further research in gene therapy and can also help identify lentivirus titer.
结论成功构建了带有大鼠bmp - 7基因的慢病毒载体，并实现其在HSC - T6的稳定高表达。
Conclusion Lentiviral vector carrying BMP-7 gene has been successfully constructed and maintains high expression in HSC-T6 cells.
目前应用的病毒载体主要有逆转录病毒载体、腺病毒载体、腺病毒相关病毒、慢病毒载体、单纯疱疹病毒载体等。
At present, the mainly applied virus vectors are retroviral vector, adenovirus vector adenovirus-associated virus slow virus vector, herpes simplex virus vector and so on.
目的构建表达人类白细胞抗原- E (HLA - E)基因慢病毒载体，探讨慢病毒介导HLA - E基因在肿瘤免疫中的意义。
Objective to construct the lentiviral expression vector of HLA-E gene and investigate its significance for further study on tumor immunity.
目的：通过多点突变构建增强型青色荧光蛋白(ECFP)慢病毒表达载体。
Objective: to construct enhanced cyan fluorescence protein (ECFP) lentiviral vector using the gene multipoint mutation.
目的：构建和鉴定人高迁移率族蛋白组a 1 (HMGA1)基因rna干扰慢病毒表达载体。
Objective: to construct and identify a lentiviral vector harboring RNAi sequence targeting the human high mobility group A1 (HMGA1) gene.
目的：构建和鉴定人高迁移率族蛋白组a 1 (HMGA1)基因rna干扰慢病毒表达载体。
Objective: to construct and identify a lentiviral vector harboring RNAi sequence targeting the human high mobility group A1 (HMGA1) gene.
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