探讨慢病毒介导GDNF对帕金森病的治疗作用。
To investigate the effects of lentivirus-mediated transfection of GDNF on Parkinson's disease.
分离结果表明，这是一种较为敏感的分离绵羊慢病毒的方法。
Suggesting that the procedure is a sensitive method for isolating OvLV.
目的尝试利用腺病毒介导慢病毒载体的细胞感染以提高转染效率。
Purpose To test the hypothesis that adenovirus may improve gene transfer efficiency of lentiviral vector by mediating viral entry.
在临床观察期间，实验羊仅出现轻微的与绵羊慢病毒有关的临床症状。
There were slight clinical signs relevant to OvLV infection in 5 seropositive ewes.
本发明涉及慢病毒基因组中的抑制性核苷酸信号序列或“INS”序 列。
The invention relates to inhibitory nucleotide signal sequences or 'INS' sequences in the genomes of lentiviruses.
目的：通过多点突变构建增强型青色荧光蛋白(ECFP)慢病毒表达载体。
Objective: to construct enhanced cyan fluorescence protein (ECFP) lentiviral vector using the gene multipoint mutation.
他们此病毒的特点是第一个已知的人类慢病毒根据其形态，生化和免疫学性质。
They characterized this retrovirus as the first known human lentivirus based on its morphological, biochemical and immunological properties.
目的慢病毒转染的RFB细胞静脉注入类风湿性关节炎大鼠，评价其治疗效果。
Objective To inject RF B cell transfected by lentivirus to arthritis rats, and to evaluate the curative effect.
目的：探讨慢病毒载体在白血病细胞中的基因转导效率，为白血病基因治疗提供关键依据。
Objective to investigate the gene transduction efficiency of lentiviral vector in leukemia cells to provide key basis for leukemia gene therapy.
目的：探讨慢病毒载体在白血病细胞中的基因转导效率，为白血病基因治疗提供关键依据。
Abstract: Objective to investigate the gene transduction efficiency of lentiviral vector in leukemia cells to provide key basis for leukemia gene therapy.
结论：基于慢病毒载体基因转导的高效性，该载体系统可作为白血病细胞基因转导的极好工具。
Conclusion This lentiviral vector is an excellent gene transduction system for leukemia cells because of its high gene transduction efficiency.
本发明公开了一种表达胶质源性神经营养因子的慢病毒载体及其用途，属于医学生物技术领域。
The invention discloses a lentiviral vector for expressing the glial cell line-derived neurotrophic factor and applications thereof, which belong to the field of medical biotechnology.
神经细胞至少能存活8周，在此期间GFP能持续表达，说明慢病毒能有效而稳定感染神经细胞。
Neurons could survive in the culture for at least 8 weeks, during which the GFP was persistently expressed, indicating the lentivirus could efficiently and stably infect the neurons.
目的：构建和鉴定人高迁移率族蛋白组a 1 (HMGA1)基因rna干扰慢病毒表达载体。
Objective: to construct and identify a lentiviral vector harboring RNAi sequence targeting the human high mobility group A1 (HMGA1) gene.
携带GF P报告基因的慢病毒可感染成熟脂肪细胞，感染率约为80%，且细胞被感染后状态良好。
Lentivirus with GFP reporter can infect differentiated adipocytes. The infection efficiency was about 80%, and infected cells were in good conditions.
这些慢病毒非常不同，以至于猫类不会感染HIV而人类不会感染FIV，但是他们基础的生物化学属性是相同的。
The viruses, known as lentiviruses, are different enough that cats can't catch HIV and people can't get FIV, but most of their basic biochemistry is the same.
构建成功的新型慢病毒载体为今后的基因治疗奠定了基础，也为慢病毒滴度的确定提供了一种新的可能。
This lays the foundation for the further research in gene therapy and can also help identify lentivirus titer.
目的：为分析慢病毒介导的转基因小鼠中外源基因整合位点的信息，应用反向PCR克隆整合位点序列。
Objective: To investigate the information of transgene integrations in mice mediated with lentiviral vectors, the inverse PCR was used to clone the sequences of integration sites.
结论成功构建了带有大鼠bmp - 7基因的慢病毒载体，并实现其在HSC - T6的稳定高表达。
Conclusion Lentiviral vector carrying BMP-7 gene has been successfully constructed and maintains high expression in HSC-T6 cells.
目前应用的病毒载体主要有逆转录病毒载体、腺病毒载体、腺病毒相关病毒、慢病毒载体、单纯疱疹病毒载体等。
At present, the mainly applied virus vectors are retroviral vector, adenovirus vector adenovirus-associated virus slow virus vector, herpes simplex virus vector and so on.
目的：探讨重组慢病毒介导的体外转导OPCML基因的可行性及其对卯巢上皮性癌（卵巢癌）细胞的抑制作用。
Objective: To study the inhibition of OPCML to ovarian cancer cell lines using a lentiviral vector system for efficient gene transduction.
干扰素刺激基因所编码干扰素刺激蛋白的高水平表达可以抑制病毒的复制，这种抑制是导致慢病毒建立潜伏感染的原因之一。
The over expression of interferon stimulated protein can inhibit the replication of viruses. The inhibition can lend lentivirus to establish latent infection.
本研究以牛免疫缺陷病毒（BIV）为慢病毒模型，对一种干扰素刺激蛋白ISG15在BIV潜伏感染中的作用进行了初步的探讨。
In this work, we used BIV as a lentivirus model, and did a preliminary study on the function of bovine ISG15 (bISG15) in latent infection of BIV.
目的构建表达人类白细胞抗原- E (HLA - E)基因慢病毒载体，探讨慢病毒介导HLA - E基因在肿瘤免疫中的意义。
Objective to construct the lentiviral expression vector of HLA-E gene and investigate its significance for further study on tumor immunity.
然而，当该补丁的传播速度比病毒来得慢时，它可能并不能够阻止病毒的蔓延。
However, while the patch may slow the spread of the virus it may not be enough to stop it.
慢乙肝患者抗病毒治疗的根本目标是减少远期严重并发症的发生。
The aim of antiviral treatment for HBV is to reduce the long term severe complications.
目的：观察慢性乙型肝炎（慢乙肝）患者细胞因子及病毒指标与中医证型的关系。
Objective:To explore the correlation between different syndrome types of TCM and serum cytokines and replication of Virus index in chronic hepatitis B(CHB).
目的：观察慢性乙型肝炎（慢乙肝）患者细胞因子及病毒指标与中医证型的关系。
Objective:To explore the correlation between different syndrome types of TCM and serum cytokines and replication of Virus index in chronic hepatitis B(CHB).
应用推荐