正交设计可提高试验效率及准确性,是愈伤组织诱导培养基、分化培养基筛选优化的好方法;
Orthogonal design improved experimental efficiency and precision, which was a good way for selection and optimization of callus induction medium and differentiation medium.
对于愈伤组织的再分化,不仅需要细胞分裂素,而且诱导培养基中生长素浓度的影响也是显著的。
With regard to the callus redifferentiation, not only kinetin is needed, but also the effect of auxin concentration of the induction medium is also remarkable.
在MS培养基中加入不同植物激素,研究黑果腺肋花楸离体叶片愈伤组织的诱导及其再分化。
Callus induction and redifferentiation of black chokeberry leaf in vitro were studied when a diversity of plant hormones were added to MS media.
采用正交设计方法,研究了NAA、6-BA、MS培养基对明党参叶片与叶柄愈伤组织诱导的影响。
Effects of NAA, 6-BA, MS culture medium on leaf and petiole callus induction of Changium smyrnioides Wolff were studied by orthogonal design.
原生质体在培养基中诱导出小愈伤组织。
利用水稻成熟胚为外植体,接种在NB培养基上,分别附加不同的外源激素,以诱导愈伤组织并促其分化,最终获得水稻再生植株。
The mature embryos were cultivated on NB media with different hormones, by using the mature embryos in rice as explants, and regeneration plants were obtained successfully.
选择合适的水稻花培起始材料,选用适宜的培养基配方,对于水稻花培中愈伤组织诱导和分化至关重要。
It was important for callus induction and shoot differentiation to choose better starting materials and a suitable medium in rice anther culture.
转化细胞来自白花曼陀罗嫩茎在LS固体培养基上诱导产生的愈伤组织。
Cultured cells derived from stems of Datura stramonium were maintained in Linsmaiher and Skoog (ls) solid medium.
以大叶黄杨的幼茎段为外植体,在添加BA和NAA、IBA、2,4-D不同激素组合的MS培养基上培养,对其愈伤组织进行诱导试验。
With young stem segment of Euonymus japonicus as explants, callus induction were studied on MS basal medium supplemented with different hormone combinations including BA and NAA, IBA , 2,4-D.
M6培养基适于侧芽生长和皮下组织诱导愈伤;
M6 medium was suitable for growth of side-bud and callus Induction of subcutaneous tissue.
方法:将丹参外植体培养在含不同激素配比的诱导培养基上,并对其愈伤组织中所含次生代谢产物进行分析。
Method:Study the development of allochtonic formations in the callus from explants of Salvia miltiorrhiza cultured on hormones of different content ratios in the medium.
以玉米自交系4112的幼胚为外植体,以MS为基本培养基,研究了不同质量浓度的2,4 - D对愈伤组织诱导的影响。
Influence of different mass concentration of 2, 4-d on callus induction in maize inbred line 4112 from immature embryos with MS being used as basal culture medium was studied.
以美国黑树莓叶片作为外植体,以MS为基本培养基,添加不同浓度的6-BA、NAA或IAA进行愈伤组织诱导及植株再生试验。
The leaves of the blackberry as the explant, and MS contained different concentration of 6-BA, NAA or IAA as the media, the callus induction and the plant regeneration of the blackberry were studied.
培养基类型对愈伤组织的诱导和分化有明显影响,MS培养基优于DCR培养基。
Media had obvious effect on calli induction and differentiation. MS medium was better than DCR medium.
结果表明,以离体去皮根段为外植体,MS为基本培养基,诱导愈伤组织较为适宜。
Callus was efficiently induced from in vitro root segment devoid of rhizodermis on MS medium.
以金边瑞香茎段为外植体,探讨了不同培养基对愈伤组织诱导、愈伤组织分化的影响。
These young stems as explants placed on different media formed calli, whose amount then increased by different cultures on different media and for different generations.
在不添加2,4 - D的培养基中,外植体的愈伤组织诱导率很低,并且需要诱导很长的时间。
Without added 2, 4-d in the medium, explant callus induction rate is very low, and needed a very long time to induct.
苔段在含盐培养基上均不能诱导出愈伤组织。
The callus from stem segments can not induced in the medium added to NaCl.
W14 是适合花药愈伤组织诱导的基本培养基;
W14 was the best basal media for the first step of callus induction.
取继代培养30 d的铁皮石斛类原球茎作接种材料,以N6为基本培养基,加入植物激素,并以真菌提取物作诱导子,对类原球茎和愈伤组织进行诱导培养。
Methods Protocorm-like bodies and callus of DCWL subcultured for 30 days was used as the explants, N6 was used as the basic culture with phytohormone added, and fungal extracts as the elicitor.
胚性愈伤组织转移到体细胞胚诱导培养基中可诱导出体细胞胚。
Somatic embryos could be induced after the embryogenic calli were transplanted on somatic embryo induction medium.
红枫腋芽在培养基上诱导出愈伤组织并增殖,但未分化;
The bud can easily produce calli and propagate but has no differentiation on the culture medium.
结果表明:CC培养基是绝大多数水稻愈伤组织的最适诱导与继代培养基;
The main results were as following: For most rice CC medium was the best for both callus initiation and subculture.
在愈伤组织诱导过程中,比较了不同基本培养基、不同浓度的激素组合、不同外植体的影响。
In the course of calli induction, different basal media, combination of plant growth regulators and explants were compared.
试验还得到唐菖蒲“超级玫瑰”球茎诱导愈伤组织的最佳培养基和愈伤组织生长的最适培养基。
The effects of electron beam and fast neutrons on the callus induction of corm of gladiolus"Rose Supreme"were investigated by using the orthogonal experiment design.
结果:将茎段愈伤组织接种在含有0。2%秋水仙素的培养基上,通过培养形成的多倍体植株诱导率为12。
Result: The polyploid of P. chinense was obtained using calli with stem treated with 0.2% colchicines medium, the inducing rate reached 12.45%.
实验结果表明:以MS为基本培养基,在愈伤诱导培养基中加入2,4-D利于愈伤组织的诱导;
The experimental results show that when adopting MS basal medium, adding 2,4-D benefit to induce callus;
实验结果表明:以MS为基本培养基,在愈伤诱导培养基中加入2,4-D利于愈伤组织的诱导;
The experimental results show that when adopting MS basal medium, adding 2,4-D benefit to induce callus;
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