花蕾的发育时期是影响愈伤组织发生的重要因素。
小麦成熟胚愈伤组织的诱导率、生长率及胚性愈伤组织发生率的基因型效应有显著差别。
The effect of genotype on the rate of induction, growth and embryogenic callus initiating were different significantly.
二是先诱导获得愈伤组织，再经继代培养获得胚胎发生。
Embryogenic callus and somatic embryos could be directly obtained on the medium only with ZT, but it was influenced by explant sources.
同时，利用细胞学和扫描电子显微镜技术观察了愈伤组织形成和器官发生过程。
Meanwhile, the processes of callus formation and organogenesis were observed by means of cytological and scanning electron microscopic techniques.
同时，运用石蜡切片法研究剥皮后愈伤组织的形成、木栓形成层和维管形成层的发生；
Meanwhile, paraffin-cut section method was used for studying on callus formation, cork cambium and vascular cambium initiation.
测定了龙眼胚性愈伤组织体胚发生过程中若干生物大分子的动态变化。
The dynamic changes of some biomacromolecules were measured in the process of somatic embryogenesis from embryogenic callus in longan(Dimocarpus longan Lour. ).
对棉花体细胞胚胎发生和植株再生基因型范围的拓宽进行了研究，有望得到丰产抗病棉花品种农大94 - 7的胚性愈伤组织。
To broad the number of regenerable cotton species, a new high yield and disease resistant variety, Nongda94-7 was studied, and Embryogenic callus of it could be induced for the future.
研究结果表明，新型植物生长调节剂TDZ有利于棉花愈伤组织的诱导，但不利于棉花愈伤组织的增殖和保持，也不利于棉花体细胞胚的发生。
TDZ was advantageous to induction of cotton callus, but it was not advantageous to proliferation and maintain of callus, and was not advantageous to induction of somatic embryoids.
以苏丹草幼穗为外植体材料，研究幼穗发育期以及配比的激素组合对愈伤组织诱导发生、生长状态及其绿苗分化能力的影响。
Effect of development period of immature inflorescences and hormone compositions of different kinds and ration on callus induction, growth state and plant regeneration was studied.
据此我们认为在甘蔗组织培养中2.4- D可能通过调节内源激素的水平及其相互作用，引起培养物中某些生理生化过程发生改变，从而进行脱分化和愈伤组织形成。
It appeared that the sugarcane leaf segments dedifferentiation and callus formation induced by 2.4-d May be related with the changes of endogenous levels of zeatin, zeatin riboside and ABA.
该培养基适于培养大豆等豆科植物未成熟子叶原生质体，通过胚性愈伤组织，经胚胎发生途径。
This callus can differentiate somatic embryo and regenerate plant on the culture medium somatic embryo differentiation.
实验中，利用石蜡切片法和扫描电镜技术对愈伤组织的发生及细胞整体形貌进行了观察。
At the same time, the callus occurrence and cell configuration of the callus were observed via paraffin slice method and scanning electron microscope technology.
因时间有限，没有系统地研究杂交松体胚发生的完整过程，如何促进愈伤组织的分化及体细胞胚的诱导有待进一步的深入研究。
We were not able to induce somatic embryos because of limited time. How to make the callus differentiate and induce the somatic embryos was the basis of embryogenesis in future.
因时间有限，没有系统地研究杂交松体胚发生的完整过程，如何促进愈伤组织的分化及体细胞胚的诱导有待进一步的深入研究。
We were not able to induce somatic embryos because of limited time. How to make the callus differentiate and induce the somatic embryos was the basis of embryogenesis in future.
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