来自抗病无性系的愈伤组织也表现抗病。 愈伤组织抗病性表达受培养基的激素浓度及比例、病原菌的接种量和接种温度影响较大;
The expression of poplar disease resistance in callus tissues is greatly affected by concentration of auxins and cytokinins in the medium, inoculum density and temperature.
利用水稻成熟胚为外植体,接种在NB培养基上,分别附加不同的外源激素,以诱导愈伤组织并促其分化,最终获得水稻再生植株。
The mature embryos were cultivated on NB media with different hormones, by using the mature embryos in rice as explants, and regeneration plants were obtained successfully.
本研究对甘蔗愈伤分化过程进行了观察,并对分化过程中的内源激素进行了测定、分析。
This paper reported studies on the differentiation of sugarcane callus and the changes of endogenous hormones in the course of differentiation.
方法:将丹参外植体培养在含不同激素配比的诱导培养基上,并对其愈伤组织中所含次生代谢产物进行分析。
Method:Study the development of allochtonic formations in the callus from explants of Salvia miltiorrhiza cultured on hormones of different content ratios in the medium.
因此,认为植物产生愈伤组织和再分化为植株不仅与外源激素有关,而且因植物材料和营养条件而异。
Therefore, it is concluded that regulation of callus formation and differentiation of the calli by exogenous hormone is closely related to some endogenous factors and external condition.
以大叶黄杨的幼茎段为外植体,在添加BA和NAA、IBA、2,4-D不同激素组合的MS培养基上培养,对其愈伤组织进行诱导试验。
With young stem segment of Euonymus japonicus as explants, callus induction were studied on MS basal medium supplemented with different hormone combinations including BA and NAA, IBA , 2,4-D.
研究了品种、基本培养基、激素处理、愈伤组织切块大小对红掌愈伤组织分化培养的影响。
The influences were studied of variety, basic medium, hormone combination and slice block of callus on the callus differentiation culture of Anthurium andraeanum.
可见,基因型对小麦愈伤组织的分化有很大的影响,附加一定的外源激素有利于提高植株的再生频率。
So the genotypes have great influence on wheat callus differentiation and to add certain amount of hormone could increase the rate of plant regeneration.
内源激素的种类和水平调控着愈伤组织的分化。
The kinds and levels of endogenous hormones modulate the differentiation of callus.
对不同外植体及其愈伤组织的内源激素含量进行分析发现,内源激素的含量和配比是影响愈伤组织诱导率和绿苗分化率的关键因素之一。
Through analyzing the endogenous hormones content of different explants and calli, the content and mixture ratio of endogenous hormones were one of the key factors that can affect CEF and GPDF.
外源生长素和细胞分裂素是诱导离体培养细胞分化与增殖所必需的,2,4-D是诱导胚性愈伤组织的重要激素。
The exogenous auxin and cytokinin are necessary to induced differentiation and proliferation of cells of culture in vitro. 2,4-D is an important hormone of induced embryogenic calluses.
由此看来,胚性愈伤组织的诱导和保持,可能还与各种激素的协同作用有关。
By this token, induction and subculture of embryonic calli should be related with cooperative effects among different hormones.
其它的植物激素如2,4 - D,NAA和KT等对三七愈伤组织分化影响不大,但亦改变愈伤组织的生长状态。
Other plant hormones such as 2, 4-d, NAA or KT did not influenced obviously the callus differentiation, but they could change the growth state of the callus.
利用粉枝莓的茎段和幼嫩叶片作为外植体,研究了不同激素水平对诱导产生愈伤组织、芽和根,完成植株再生的影响。
Using stems and young leaves of Rubus biflorus as explants, this paper researched the effect on inducing callus, budlets and roots and plantlet regeneration of different concentration hormone.
在MS培养基中加入不同植物激素,研究黑果腺肋花楸离体叶片愈伤组织的诱导及其再分化。
Callus induction and redifferentiation of black chokeberry leaf in vitro were studied when a diversity of plant hormones were added to MS media.
水稻遗传转化过程中,内源激素的含量及变化影响着愈伤组织的生理状态。
In the process of rice genetic transformation, the levels and changes of endogenous phytohormones affect calli's physiological status.
以苏丹草幼穗为外植体材料,研究幼穗发育期以及配比的激素组合对愈伤组织诱导发生、生长状态及其绿苗分化能力的影响。
Effect of development period of immature inflorescences and hormone compositions of different kinds and ration on callus induction, growth state and plant regeneration was studied.
添加激素i和暗培养有利于愈伤组织形成色素,诱导率达30%。
Adding of hormone I in dark culture, it was favourable to the pigments formation in callus. The inducement radio was 30%.
据此我们认为在甘蔗组织培养中2.4- D可能通过调节内源激素的水平及其相互作用,引起培养物中某些生理生化过程发生改变,从而进行脱分化和愈伤组织形成。
It appeared that the sugarcane leaf segments dedifferentiation and callus formation induced by 2.4-d May be related with the changes of endogenous levels of zeatin, zeatin riboside and ABA.
结果表明,激素对子叶切块愈伤组织诱导的影响是很明显的。
The results show that the impact of hormone on callus induction from cotyledon is obvious.
激素和蔗糖的浓度对百脉根愈伤组织的出愈率有很大的影响。
The concentration of hormone and sugar has a great impact on the wound curing rate of the callus of Lotus.
胚性愈伤诱导率和植株再生率与培养基的种类、培养基中2,4 - D的浓度、小麦基因型及诱导和分化培养基中激素种类和浓度等有关。
Callus formation frequency as well as plantlet regeneration frequency was dependent on the composition of basal medium and the concentration of 2, 4-d.
在含有激素的培养基上,离体胚形成大量愈伤组织和畸形苗。
The embryo formed into a lot of callus and transformed into teratosis plants when were cultured on MS with hormone.
结果表明,龙眼胚性愈伤组织中内源激素含量比非胚性愈伤组织高;
The results showed that the levels of endogenous phytohormones in embryogenic calli were higher than those in non-embryogenic calli.
结果表明,愈伤组织诱导率和植株再生与水稻的成熟胚及其愈伤组织的内源激素含量有关。
The results showed that the callus initiation and plant regeneration were related to endogenous hormones of mature rice embryo and its derived callus.
取继代培养30 d的铁皮石斛类原球茎作接种材料,以N6为基本培养基,加入植物激素,并以真菌提取物作诱导子,对类原球茎和愈伤组织进行诱导培养。
Methods Protocorm-like bodies and callus of DCWL subcultured for 30 days was used as the explants, N6 was used as the basic culture with phytohormone added, and fungal extracts as the elicitor.
碳源、氮源、植物激素对愈伤组织生长和黄酮类形成影响较为显著。
Carbon source, nitrogen source and plant hormone had the obvious effects on the callus growth and flavonoids production.
在愈伤组织诱导过程中,比较了不同基本培养基、不同浓度的激素组合、不同外植体的影响。
In the course of calli induction, different basal media, combination of plant growth regulators and explants were compared.
本实验通过对基本培养基、蔗糖浓度、激素配比、基因型的因素的筛选对苎麻花药培养愈伤诱导体系进行了优化。
We have optimized the ramie anther culture method by selecting the basic media, sucrose concentration, plant growth regulators and genotypes.
本实验通过对基本培养基、蔗糖浓度、激素配比、基因型的因素的筛选对苎麻花药培养愈伤诱导体系进行了优化。
We have optimized the ramie anther culture method by selecting the basic media, sucrose concentration, plant growth regulators and genotypes.
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