利用余下的521对多态性引物获得634 个标记位点。
The rest 521 polymorphic primer pairsamplified 634 marker loci.
目的：研究建立等位基因特异性引物pcr技术体系，并将其应用于基因单核苷酸多态性研究工作。
Objective: to study and establish an allelic specific primer polymerase chain reaction (ASP-PCR) technique system and to apply this technique to study single nucleotide polymorphism (SNP) of genes.
筛选出25个多态性引物构建了41份甘蔗种质的RAPD指纹图谱，并对RAPD数据进行聚类分析。
RAPD fingerprints of 41 sugarcane germplasms were structured using 25 selected polymorphic primers, and the RAPD data were analysed.
目的：(1)建立使用等位基因特异性引物方法检测KLOTHO基因单核苷酸多态性的PCR反应体系。
Objective: (1) to establish PCR reaction system that USES allele-specific primer PCR technique to detect SNP of KLOTHO gene.
这31条多态性引物和所检测到的93个多态性位点初步为草鱼基因组的多态性分析提供了可靠的分析指标体系。
Both the 93 polymorphic loci and the 31 polymorphic primers might provide a reliable molecular marker system for grass carp genomic DNA polymorphism analysis.
利用本实验室自行设计的梨火疫病菌特异性引物REA-FEA，再次验证了免疫吸附-PCR技术的灵敏性和专化性，得到了同样的结果。
Using the specific primers REA-FEA designed in this work by ourselves, we proved the specificity and sensitivity of the immuno-capture-PCR method again. The same result has been achieved.
在51条随机引物中筛选出具有稳定扩增、条带清晰的多态性引物16条，16条引物共检测到132个位点，其中多态位点114个（86.36%）。
RAPD primers were synthesized and 16 primers were selected and used. A total of 132 loci were amplified, of which 114 loci(86.36%) were polymorphic.
本实验利用种属相似性设计了一对引物。
In the experiment, a pair of primers was designed by species similarity.
每对引物可扩增出50条左右的条带，其中，多态性条带数为12.4条。
The bands amplified by each primer combination were 50, in which the polymorphism bands was 12.4.
在应用这一方法时，我们最关心以下三个方面：敏感性，忠实性和引物设计的方便。
Our main concern while using this method involves sensitivity, fidelity and the ease of primer design.
结论采用通用性强的引物系统配合特异性高的热启动PCR技术检测临床和实验室标本中是否存在病原性真菌的方法有重要的应用潜力。
Conclusion This highly universal primer system in combinaition with highly specific hot initiated PCR might be used in the detection of medically important fungi in experimental or clinical specimens.
同时筛选得到14个扩增稳定、多态性丰富的ISSR引物。
And 14 primers with stable amplification and rich polymorphism for ISSR were screened.
方法：荧光标记的多态性微卫星引物d10s1265与83例结直肠癌的肿瘤和正常组织进行PCR反应。
Methods: D10S1265, a fluorescent labeled polymorphic microsatellite marker, was analyzed in 83 cases of sporadic CRC and normal tissue DNA by PCR.
结果筛选出的33个随机引物对68个柞蚕品种（系）共扩增出296条DNA带，其中多态性带269条（90.88%）。
ResultA total of 296 DNA fragments were amplified with 33 primers selected from 68 cultivars of A. pernyi, 269 of which (90.88%) were polymorphic.
说明荔枝绝大多数SSR引物对龙眼属植物具有适用性。
It showed that a large number of SSR primers from litchi had applicability to Longan.
每对引物扩增的等位基因数在3 ~13个，平均每个位点有5.6个等位基因，显示了较高的多态性。
The allele Numbers for each primer ranged 3 to 13, and a mean of 5.6 alleles were found for each locus, which indicated a higher polymorphism.
利用ISSR标记对15份葡萄材料进行了基因组多态性分析，从60条引物中筛选出15条用于葡萄的ISSR扩增。
Vitis germplasm were used as materials for analyzing their genome polymorphism by ISSR markers. 15 primers selected from 60 primers were used for ISSR amplification.
采用脉冲场凝胶电泳（PFGE）和随机引物多态性DNA（RAPD）分型对128株CRAB进行分子流行病学研究；
The homology of 128 isolates of CRAB was determined by both pulsed field gel electrophoresis (PFGE) and randomly amplified polymorphism DNA (RAPD).
对12份莲雾资源和2个莲雾近缘种进行了ISSR分析，18条引物共扩增出459个位点，其中多态性位点435个（94.77%）。
ISSR analysis with 18 primers to 12 wax apple(Syzygium samarangense Merr. et Perry)germplasm and 2 closely related species produced 459 unambiguous bands, out of them 435(94.77%) were polymorphic.
利用SSR引物的种间通用性，本研究分别从杨属和柳属中各随机筛选出10对SSR引物，对17个杨树品种和20个柳树品种的遗传关系进行了分析。
In this research, random screening of 10 SSR primers from Populus and Salix, respectively, and the genetic relationship among 17 poplar cultivars and 20 willow cultivars were studied.
重组体用克隆位点上游和下游的T7和T3启动子序列为测序引物，用自动测序仪测序鉴定克隆的正确性。
The recombinant was sequenced by automatic sequencer with promoters T7 in upstream and T3 in downstream as sequencing primers.
所分析的20个随机引物共扩增出了109条带，但是只有引物s303、S4出现4个多态位点，多态性位点所占的比例(P)只有3.7%。
Among 109 loci revealed by 20 RAPD primers, only primer S303 and S4 showed 4 polymorphic loci. The percent of polymorphic loci (p) was only 3.7%.
目的探讨引物原位标记法（PRINS）结合经腹脐血穿刺，应用于脐血中期细胞快速产前诊断18号染色体数目异常的可行性。
Purpose To study the feasibility of PRINS for rapid prenatal diagnosis of fetal 18 aneuploidy in metaphase cells from umbilical blood.
其RNA依赖的RNA聚合酶活性有一定的引物依赖性。
Its RNA polymerase activity was dependent upon the primer in vitro with limited reverse transcription activity.
选用分布于水稻12条染色体上的40对SSR引物对6个杂交水稻组合及其亲本的幼苗进行了SSR等位基因多态性分析。
SSR allele polymorphism of 6 hybrids and their parents was studied by analysing 40 SSR loci distributing on 12 chromosomes in rice.
结果：用10个随机引物共扩出110条清晰条带，其中多态性条带为106条，总的多态位点百分率为96.36，平均多态位点百分率为38.92。
Result: Total polymorphic loci percentage was 96.36 and the average was 38.92.110 bands were produced with 10 random primers and 106 were polymorphic.
W4和W6是单引物扩增产物，与已知序列无同源性。
W4 and W6 derived from single primer amplification, and had no homology to the known sequences.
结果表明，有13对引物能获得稳定的特异性条带（占总数的50%），其中有6个微卫星位点具有多态性（占总数的23.1%）。
Electrophoresis analysis showed that 13 pairs of primers produced stable and specific bands(50%), and, of which 6 were polymorphic(23.1%).
所得的数据表明在所选的24个RAPD引物和20个ISSR引物中，培育两年的愈伤组织和它们的再生植株表现出适中水平的基因组多态性(分别为20.83%和17.04%)。
Data showed that two-year-old calli and their regenerated plants, as analyzed with 24 RAPD and 20 ISSR primers, manifested moderate levels of genomic variations (20.83% and 17.04% respectively).
因此与SRAP相比，SSR具有稳定性好、多态性高和引物特异性强等特点。
Compared with SRAP, SSR has a good stability, high polymorphism and primers specificity, and so on.
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