目的探讨脉冲微交流电刺激对体外诱导大鼠骨髓间充质干细胞向心肌样细胞分化的作用。
Objective To investigate the effects of electrical stimulation on the differentiation of rat bone marrow mesenchymal stem cells (MSCs) to cardiomyocyte induced by 5-azacytidine in vitro.
目的探讨脉冲微交流电刺激对体外诱导大鼠骨髓间充质干细胞向心肌样细胞分化的作用。
Objective to investigate human umbilical cord mesenchymal stem cells (MSCs) differentiated into cardiomyocytes, and find a new source of cells for transplantation of cardiomyocytes.
结果大鼠骨髓基质细胞贴壁呈集落生长，5 -氮胞苷诱导骨髓基质细胞转化为心肌样细胞。
Results Mesenchymal stem cells were spindle-shaped and active proliferation capacity in vitro, they can be differentiation into cardiac-like muscle cells after adding 5-azacytidine.
目的：观察脐血间充质干细胞经5-氮杂胞苷诱导在体外定向分化为心肌样细胞的形态学及分子生物学特征。
AIM: To study morphological and molecular biological feature of differentiation of umbilical cord blood stem cells in vitro after induced by 5-azacytidine into cardiomyocytes.
幼鼠骨髓干细胞经体外培养、5 -溴脱氧尿核苷标记和5 -氮杂胞嘧啶核苷诱导能形成幼稚的心肌样细胞。
Bone marrow stem cells of young rats can form immature myocardial-like cells after in vitro culture labeling of 5-bromodeoxyuridine and induction of 5-azacytidine.
方法制备心肌组织缺氧损伤模型，观察不同剂量的地高辛抗血清对缺氧损伤心肌组织内源性洋地黄样因子水平和心肌细胞膜atp酶活性的影响。
Methods it was observed that different concentration anti_digoxin antiserum effect on endogenous digitalis_like factor and cell membrane ATPase activity in hypoxic myocardium model.
细胞凋亡可降低动脉粥样硬化斑块的稳定性，增加不稳定性心绞痛和急性心肌梗死的危险性。
Apoptosis can make atherosclerotic plaque unstable, which increases the risk of unstable angina and acute myocardial infarction.
结论：骨髓间质干细胞分化为心肌细胞过程中，随培养时间延长及细胞密度增加逐渐形成闰盘样结构。
CONCLUSION: During the process of BMMSCs differentiating into cardiomyocytes, intercalated disc-like structure is gradually formed with increase in culture time and cell density.
结论：骨髓间质干细胞分化为心肌细胞过程中，随培养时间延长及细胞密度增加逐渐形成闰盘样结构。
CONCLUSION: During the process of BMMSCs differentiating into cardiomyocytes, intercalated disc-like structure is gradually formed with increase in culture time and cell density.
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