外源淀粉水解酶能在酵母中高效表达并分泌,其主要因素在于:在构建载体时组入强启动子;
The main factors for high efficiency expression and secretion of exogenous amylum hydrolase in S. cerevisiae are as follows: involvement of strong promoter in carrier construction;
结果表明,获得的强启动子比原来的提高了3 ~8倍,而弱启动子则活性下降明显,其中3个几乎无活性。
Results showed that the activity of strong promoters had been improved 3 ~ 8 fold while one weak promoter had been decreased 3 fold and three weak promoters showed almost no activity.
结果表明,获得的强启动子比原来的提高了3 ~8倍,而弱启动子则活性下降明显,其中3个几乎无活性。
Results showed that the activity of strong promoters had been improved 3 ~ 8 fold while one weak promoter had been decreased 3 fold and three weak promoters showed almost no activity.
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