方法采用多对型特异性引物-聚合酶链反应检测160例慢性乙型肝炎患者血清HBV基因型；
Methods: Serum samples from 160 cases with chronic HBV infection were collected and tested for HBV genotypes by type-specific primers.
目的建立应用任意引物聚合酶链反应进行真菌菌型鉴定的方法。
Objective To establish the method to identify fungus using the arbitrarily primed polymerase chain reaction (APPCR).
目的：应用内转录间隔区(its)通用引物建立一种较为快速、简便的检测和鉴定念珠菌的聚合酶链反应(PCR)方法。
Objective: To set up a rapid and simple PCR assay for Candida detection and identification by using internal tran scribed spacer (ITS) universal primers.
方法运用序列特异性引物聚合酶链反应技术，检测无亲缘关系湖北汉族健康人136例、食管癌组42例患者的HLA-DQB1等位基因。
METHODS HLA-DQB1 gene polymorphisms were typed by sequence specific primer based polymerase chain reaction, in 42 patients with esophageal neoplasm and 136 normal control subjects.
并把分型结果与采用聚合酶链反应-序列特异性引物（PCR-SSP）获得的结果进行比较。
The results of genotyping were compared with those obtained with the polymerase chain reaction method using sequence specific primers ( PCR-SSP).
目的筛选出最佳随机引物并优化其反应体系用于8种细菌的随机引物聚合酶链反应（AP PCR）分析。
OBJECTIVE To screen the primers and optimize the reaction systems for arbitrarily primed polymerase chain reaction(AP-PCR) on 8 sorts of bacteria.
分别设计MSX1、PAX9基因特异性引物，聚合酶链反应扩增全部外显子编码区和内含子-外显子剪接序列，产物纯化后直接测序。
Specific primers were designed for MSX1 and PAX9 respectively. Mutation analysis was performed by direct sequencing of all the coding exons and intron-exon boundaries.
方法对医院临床分离的8株耐亚胺培南铜绿假单胞菌进行随机引物聚合酶链反应（PCR）扩增，扩增产物进行电泳和聚类分析。
METHODS The genes of imipenem-resistant P. aeruginosa were amplified by RAPD assay in 8 clinical isolates and PCR products were analyzed by agarose gel electrophoresis and cluster analysis.
方法运用聚合酶链反应-序列特异性引物（PCR-SSP）法，对38例山东汉族人GPP与94例健康对照进行HLA-DQB1等位基因分型。
Methods The distributing frequencies of HLA-DQB1 alleles were detected with polymerase chain reaction-sequence specific primers (PCR-SSP) in 38 GPP patients and 94 healthy subjects from Shandong.
方法利用聚合酶链反应-序列特异引物(PCR -ssp)法，对189例银屑病患者和273例健康人的HLA - DQA1和DQB1等位基因进行检测。
Method Polymerase chain reaction sequence specific primers (PCR-SSP) method was used to analyze the frequencies of HLA-DQA1 and DQB1 alleles among 189 patients with PV and 273 healthy controls.
目的采用顺序特异引物聚合酶链反应（PCR -SSP）建立人类白细胞抗原DR位点的DNA分型方法。
Objective To establish DNA typing for HLA-DR antigens by polymerase chain reaction with sequence-specific primers (PCR-SSP).
方法：采用序列特异性引物聚合酶链反应的方法对汉族72例uc患者和314例正常对照者HLA - DRB1基因分型。
Methods: By using polymerase chain reaction-sequence specific primers, frequency of HLADRB1 alleles in 72 patients with UC and 314 healthy controls were detected.
方法：以引物互为模板的聚合酶链反应合成目的基因片段。
Methods: The PCR technique in which the primers were used as template each other was adopted to obtain the foreign DNAs for Cloning.
方法以一段特异性通用引物并配合热启动聚合酶链反应技术来检测临床标本中的棘阿米巴原虫。
Methods We used a hot initiated PCR based method with asset of universal acanthamoeba specific primers to detect acanthamoeba.
目的采用顺序特异引物聚合酶链反应技术(PCRSSP)，建立汉族人群HLAB40交叉反应组高分辨度dna分型方法。
Objective To establish a method of high resolution DNA typing for HLA B40 cross reactive groups (CREG) in Chinese with polymerase chain reaction with sequence specific primers (PCR SSP).
方法 ：根据上述抗性基因保守区域选用引物经聚合酶链反应检测成都地区临床分离78株耐 药葡萄球菌与临床 药敏试验比较并追踪其预后。
Methods:78 strains of clinically isolated staphylococci were detected by specific primers in conserved regions of mecA, ermA, ermC genes and compared with MIC determination.
方法采用聚合酶链反应(PCR)方法，用自行设计的特异引物，对昆虫的钠离子通道基因进行扩增。
Methods PCR method was applied to detect the KDR mutation of the target site (sodium channel) gene.
方法采用聚合酶链反应(PCR)方法，用自行设计的特异引物，对昆虫的钠离子通道基因进行扩增。
Methods PCR method was applied to detect the KDR mutation of the target site (sodium channel) gene.
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