引物设计是P CR技术中最重要的一环。
PCR-primer design is the most important step in polymerase chain reaction (PCR) technique.
进化DNA数据的引物设计序列统计软件。
SeqState - primer design and sequence statistics for phylogenetic DNA data sets.
关于引物设计的其他需求，可以联系作者。
The authors may be contacted regarding other requirements for primer design.
同时，对引物设计过程中常见问题进行了讨论并给出了解决办法。
In addition, common questions encountered in primer design were discussed and the corresponding measures were put forward.
在应用这一方法时，我们最关心以下三个方面：敏感性，忠实性和引物设计的方便。
Our main concern while using this method involves sensitivity, fidelity and the ease of primer design.
这里，给出了亚硫酸氢盐转换，引物设计和PCR条件优化的一个详细的实验方案。
Here, a detailed protocol for bisulphite conversion, primer design, and optimization of PCR conditions is given.
结论从引物设计的过程中可以看到一种趋势-后期引物设计的成功率远远高于早期。
Conclusions There is a trend that can be recognized in the process of designing primer. Namely, the forepart is far more than the anaphase in the ratio of success to design primer.
引物设计是RAMP和REMAP标记分析的关键，二者都使用锚定的微卫星序列引物。
The primer design is a key step for RAMP and REMAP, in which anchored simple sequence repeat primers were used.
图1抗性基因STV11引物设计策略红色表示引物结合位点，蓝色表示序列差异位点。
Fig. 1. Design strategies for STV11 marker. Primer binding sites are shown in red, sites with different sequence are shown in blue.
新方法扩展了“通用引物”的适用范围，并为引物设计和一些其它基因的PCR放大提供了思路。
Our method extended the utility of universal primers and provided implications for primer design and PCR amplification of some other genes.
通过引物设计、P CR、基因克隆、测序，获得了10种鸢尾属植物和1个外类群种的ITS序列。
ITS sequences of ten kinds of Iris plants and an outgroup were obtained by primer design, PCR, gene cloning, sequencing and cluster analysis.
本项研究经pcr引物设计后，以大熊猫和朱鹮的基因组d NA为模板，克隆了大熊猫和朱鹮的GDNF成熟蛋白基因。
Based on the GDNF gene sequence of human and chick, we cloned partial DNA sequences encoding mature protein of GDNF from genomic DNA of giant panda and crested ibis through PCR amplification.
该文就半定量RT-PCR法的检测步骤及技术的关键因素（总RNA提取、引物设计、循环数的确定和内参的选择等）进行综述。
This review summarized the recently research process of Sq RT-PCR. It including extraction of total RNA, primer design, definition of cycle count, selection of inner reference and so on.
序列信息具有实际意义-如，通过设计PCR引物，我们可以快速对这种病毒做出诊断，从而扩宽和加快监控。
Sequence information can have practical significance - for example, in designing PCR primers, to make rapid diagnosis tests that broaden and speed up surveillance.
本实验利用种属相似性设计了一对引物。
In the experiment, a pair of primers was designed by species similarity.
整合图形化序列特征，进一步协助您设计引物。
Integrated graphic sequence feature viewer to assist primer design.
对其余的15个微卫星设计引物，有10对引物扩增出目的片段。
Designed the primers of the other 15 new microsatellites, and 10 primers could obtain targeted fragments by PCR amplification.
成功进行简并PCR的关键是设计好两组引物库和对反应条件进行优化。
The optimization of reaction conditions and the primer design are the key factors for the success accomplishment of degenerate PCR.
本研究设计并合成了一对引物。
根据苯酚羟化酶基因高度保守序列设计一对该基因的特异引物。
A pair of specific primers of gene encoding phenol hydroxylase was designed by oligonucleotide high conservative sequence.
进一步工作是设计特异性引物，将其转化为SCAR标记。
Next work is to design the differential primers and transvert it to SCAR.
经设计通用引物、PCR扩增、克隆和测序，首次从分子水平鉴定了杂草赛葵上的双生病毒。
We identified the geminivirus in Malvastrum coromandelianum from the molecular level by designing the primers, PCR, cloning and sequencing.
根据菌属基因序列设计PCR引物，提取基因组dna进行PCR快速检测，然后进行测序比对。
According to the bacteria gene sequence, we also designed PCR primer, and collected genome DNA for PCR rapid test, then tested and contrasted sequence.
方法根据HCVH病毒株序列设计、合成序列特异性的引物。
Methods Sequence specific primers were designed and synthesized according to the HCV H strain of virus sequence.
方法设计相应的引物，用RT _ PCR，基因克隆，DNA序列分析技术。
MethodsThe corresponding designed primers, RT_PCR, gene cloning, DNA sequencing analysis were used.
方法根据APP的基因序列，设计并合成引物和荧光标记探针。
Methods According to the specific sequence of APP genes, the primers and the fluorogenic probe were designed and synthesized.
方法根据已报道的若干物种的NADH脱氢酶亚基4基因(nd4)设计引物。
Methods Primers were designed according to certain species' NADH-ubiquinone oxidoreductase ND4 in which the DNA sequence had been reported.
设计了特异性较好的引物。
重叠延伸PCR技术成功的关键是重叠互补引物的设计。
The overlap primers design is the key factor for the successful accomplishment of SOE PCR.
重叠延伸PCR技术成功的关键是重叠互补引物的设计。
The overlap primers design is the key factor for the successful accomplishment of SOE PCR.
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