引物的设计很关键，特异的引物序列设计参照文献。
Primer design is the key to our success, the primer sequences have designed specifically according to the reference.
重叠延伸PCR技术成功的关键是重叠互补引物的设计。
The overlap primers design is the key factor for the successful accomplishment of SOE PCR.
它不但能进行常规PCR引物、杂交探针和测序引物的设计，同时，它还能进行多重pcr引物分析以及高通量的引物搜索和查找功能。
In addition to supporting conventional applications for PCR, hybridization and sequencing, NoePrimer provides advanced capabilities for multiplex PCR and high-throughput primer search and analysis.
对其余的15个微卫星设计引物，有10对引物扩增出目的片段。
Designed the primers of the other 15 new microsatellites, and 10 primers could obtain targeted fragments by PCR amplification.
根据苯酚羟化酶基因高度保守序列设计一对该基因的特异引物。
A pair of specific primers of gene encoding phenol hydroxylase was designed by oligonucleotide high conservative sequence.
经设计通用引物、PCR扩增、克隆和测序，首次从分子水平鉴定了杂草赛葵上的双生病毒。
We identified the geminivirus in Malvastrum coromandelianum from the molecular level by designing the primers, PCR, cloning and sequencing.
新方法扩展了“通用引物”的适用范围，并为引物设计和一些其它基因的PCR放大提供了思路。
Our method extended the utility of universal primers and provided implications for primer design and PCR amplification of some other genes.
方法设计相应的引物，用RT _ PCR，基因克隆，DNA序列分析技术。
MethodsThe corresponding designed primers, RT_PCR, gene cloning, DNA sequencing analysis were used.
成功进行简并PCR的关键是设计好两组引物库和对反应条件进行优化。
The optimization of reaction conditions and the primer design are the key factors for the success accomplishment of degenerate PCR.
在应用这一方法时，我们最关心以下三个方面：敏感性，忠实性和引物设计的方便。
Our main concern while using this method involves sensitivity, fidelity and the ease of primer design.
引物设计是RAMP和REMAP标记分析的关键，二者都使用锚定的微卫星序列引物。
The primer design is a key step for RAMP and REMAP, in which anchored simple sequence repeat primers were used.
引物设计是P CR技术中最重要的一环。
PCR-primer design is the most important step in polymerase chain reaction (PCR) technique.
目的利用自行设计的引物、探针和新型铕螯合物BHHCT，建立一种半定量丙型肝炎病毒(HCV)RNA的检测方法。
Objective To establish a semi-quantitative method for measurement of HCV RNA by use of primers and probe, which was sensitive and designed by ourselves, a new europium fluorescent chelate BHHCT.
结果说明应用所设计的引物进行RTPCR快速检测PRRS是可行的，为我国快速特异诊断PRRS和PRRSV强毒株的深入研究奠定了基础。
The result showed that PRRSV can be quickly diagnosed by RT PCR, which laid a basis for quick diagnosis of PRRS and further research of the PRRSV isolates of our country.
设计了特异性较好的引物。
根据计算机克隆的ZNF322基因序列设计引物，从人类胚胎心脏文库中克隆了ZNF322基因。
The human novel gene of ZNF322 is cloned from human fetal cDNA library using the primers based on the ZNF322 sequence analyzed with computer.
根据已报道的马铃薯卷叶病毒基因组序列，设计合成一对特异性引物。
Based on the reported genomic RNA sequence of PLRV, two specific primers were synthesized.
本项研究经pcr引物设计后，以大熊猫和朱鹮的基因组d NA为模板，克隆了大熊猫和朱鹮的GDNF成熟蛋白基因。
Based on the GDNF gene sequence of human and chick, we cloned partial DNA sequences encoding mature protein of GDNF from genomic DNA of giant panda and crested ibis through PCR amplification.
方法根据HCVH病毒株序列设计、合成序列特异性的引物。
Methods Sequence specific primers were designed and synthesized according to the HCV H strain of virus sequence.
该研究通过设计合成hla基因序列特异性引物，建立了HLA -DR基因分型的套式扩增和直接扩增pcr -SSP技术，并在骨髓移植配型中进行了应用。
In this study, a nested PCR SSP and a direct amplification PCR SSP protocols for HLA DR genotyping were developed and were used in the selection of matched donor for sibling BMT.
方法根据APP的基因序列，设计并合成引物和荧光标记探针。
Methods According to the specific sequence of APP genes, the primers and the fluorogenic probe were designed and synthesized.
进化DNA数据的引物设计序列统计软件。
SeqState - primer design and sequence statistics for phylogenetic DNA data sets.
在FMDV的核苷酸序列相对保守区设计上下游引物FM1/FM4，扩增病毒的基因片段。
Then capture FMDV from clinical material with sterilized Eppendrof tubes of coating type-specific IgG, Amplify the conserved viral sequences by common primers (FM1/FM4).
根据人的GAPDH基因和毛冠鹿的钾通道基因设计引物。
We designed and synthesized the primers of the human GAPDH gene and the Elaphodus cephalophus potassium channel gene.
方法根据已报道的若干物种的NADH脱氢酶亚基4基因(nd4)设计引物。
Methods Primers were designed according to certain species' NADH-ubiquinone oxidoreductase ND4 in which the DNA sequence had been reported.
在对OPF0 2 757进行克隆、测序的基础上，设计一对PCR引物，建立了簇毛麦基因组特异性PCR标记。
On the basis of cloning and sequencing of OPF02 757 , two PCR primers were designed and a genome specific PCR marker for H.
主要进行了以下工作：（1）根据GENBANK公布的羊痘病毒基因组序列设计引物，利用PCR克隆GTPV-TY株TK基因和P32基因；
The following work were done in this study: (1)TK and P32 gene of GTPV-TY were cloned by PCR with primer pairs designed by information published on GENBANK;
新设计的引物用于两种尘螨的检测有较好的特异性和敏感性。
The new primers designed are highly specific and sensitive in detection of the two mites.
新设计的引物用于两种尘螨的检测有较好的特异性和敏感性。
The new primers designed are highly specific and sensitive in detection of the two mites.
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