通过对不同分离株弓形虫抗原的分析，探讨弓形虫虫株间的差异性。
To observe the difference among Toxoplasma strains, we analyzed antigens of various strains of Toxoplasma.
克隆与表达弓形虫缓殖子期特异性抗原1（BAG1）的基因，并分析重组抗原的免疫反应性。
Clone and express bradyzoite antigen 1(BAG1) gene of T. gondii, and analyze the immunoreactivity of the recombinant product.
结论：免疫-P CR是一种特异性强，敏感性高的弓形虫可溶性抗原检测方法。
Conclusion: Immuno PCR is a highly specific and sensitive method for the detection of Toxoplasma dissoluble Antigens.
目的 对弓形虫表面抗原SAG4基因进行克隆、表达和免疫反应性分析。
To clone and express surface antigen SAG4 gene of Toxoplasma gondii, and analyze its immunoreactivity.
目的：构建弓形虫棒状体蛋白（ROP2 ）基因重组质粒并在大肠杆菌中表达，用于筛选弓形虫新的诊断抗原和疫苗分子。
Objective:To construct a recombinant plasmid containing rhoptry protein 2(ROP2)gene of Toxoplasma gondii and express it in E. coli for selection of diagnostic antigen and vaccine candidate.
目的构建表达P30蛋白抗原表位的重组质粒及工程菌，获得纯化的P30蛋白抗原表位，用于检测弓形虫特异性抗体。
Objective To construct a recombinant plasmid containing P30 gene of Toxoplasma gondii and obtain recombinant protein Methods P30 gene fragment was amplified from genomic DNA of T.
结论通过构建基因打靶载体 ，可有目的地敲除弓形虫膜抗原基因 ，为进一步研究弓形虫侵入机制 ，探讨弓形虫病防治提供可行的方法。
These results demonstrate the utility of the gene targeting approach in the study to investigate the gene function of T. gondii. and the mechanism by which this parasite invades to the host cells.
目的克隆与表达弓形虫缓殖子期特异性抗原1（BAG1）的基因，并分析重组抗原的免疫反应性。
Objective To clone and express bradyzoite antigen 1(BAG1) gene of T. gondii, and analyze the immunoreactivity of the recombinant product.
目的克隆与表达弓形虫缓殖子期特异性抗原1（BAG1）的基因，并分析重组抗原的免疫反应性。
Objective To clone and express bradyzoite antigen 1(BAG1) gene of T. gondii, and analyze the immunoreactivity of the recombinant product.
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