采用套式聚合酶链反应方法对合并TTV感染情况进行回顾性研究。
TTV infection in the patients was retrospectively analyzed with nested PCR detection of TTV DNA in serum.
方法用半巢式聚合酶链反应方法检测72例非甲—庚型肝炎患者血清中ttv - DNA。
Methods TTV-DNA in serum of 72 patients with hepatitis Non-A to G were detected by Hn-PCR.
目的建立套式聚合酶链反应（PCR）加限制性酶切分析方法检测人巨细胞病毒（HCMV）。
Objectives To establish an nested (polymerase chain reaction) PCR analytic method with restrictive enzyme to detect human cytomegalovirus (HCMV) rapidly in clinics.
方法：应用半巢式聚合酶链反应技术检测113例孕产妇血清及其新生儿脐血的TTV—DNA。
Methods: TTV-DNA of 113 cases of parturients' blood serum and neonatal umbilical blood were detected by semi-nested polymerase chain reaction.
目的探讨单管巢式聚合酶链反应(SNPCR)检测石蜡包埋组织结核分支杆菌dna的特异性和敏感性。
Objective To study the sensitivity and specificity of single tube nested polymerase chain reaction (SN PCR) technique in detecting Mycobacterium tuberculosis DNA in paraffin embedded tissues.
方法从确诊的HIV - 1感染者全血样本中提取基因组dna，经套式聚合酶链反应(PCR)扩增和测序。
Methods Genomic DNA was extracted from the whole blood samples collected from HIV-1 positive patient. Nested PCR was carried out and PCR products were purified and used directly for sequencing.
方法采用套式聚合酶链反应（nPCR）对191例、6种不同人群血清中SENVDNA进行D亚型和H亚型分析。
METHODS SENV-D and SENV-H DNA were detected in 191 cases who were divided into 6 groups with nested-PCR.
方法应用嵌套式逆转录聚合酶链反应技术检测印记基因p57kip2、LIT1、TSSC3在人类卵母细胞及植入前胚胎的正常表达。
Methods Using nested reverse transcription-PCR to analyze the expression of P57KIP2, LIT1, TSSC3 in human oocytes and preimplantation embryos.
方法应用嵌套式逆转录聚合酶链反应技术检测印记基因p57kip2、LIT1、TSSC3在人类卵母细胞及植入前胚胎的正常表达。
Methods Using nested reverse transcription-PCR to analyze the expression of P57KIP2, LIT1, TSSC3 in human oocytes and preimplantation embryos.
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