目的: 分离培养睾丸支持细胞、骨髓基质细胞、胚胎成纤维细胞和胰腺成纤维细胞,优化培养条件,建立饲养细胞系,为干细胞体外共培养体系的建立提空充足的饲养细胞。
Objective: Sepretae Sertoli cell, BMSC, MEF, Pancreatic fibroblast and establish cell lines, constructed different feeder layers for a source as a coculture system for stem cells.
目前已建立了体外培养骨髓基质干细胞的方法,而且正在摸索进一步纯化的方法和诱导分化的条件。
Cultivation, purification, as well as induction methods have been developed and that will definitely lead to further clinical application of the cells.
本实验主要利用骨髓基质干细胞的这一特性,对其进行成软骨和成骨诱导,建立诱导和鉴定体系;
This experiment mainly used this feature of bone marrow stromal stem cells to find ways to induce them into chondrocytes and osteoblasts, and also established the induction and appraisal system;
本实验主要利用骨髓基质干细胞的这一特性,对其进行成软骨和成骨诱导,建立诱导和鉴定体系;
This experiment mainly used this feature of bone marrow stromal stem cells to find ways to induce them into chondrocytes and osteoblasts, and also established the induction and appraisal system;
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