采用超声波破碎法对啤酒废酵母残余的内源蛋白酶进行提取，并以酪蛋白为底物测定内源蛋白酶的活力。
The extraction of the residue endogenic protease of waste brewer yeast was studied with the ultrasonic cell-break method.
本文报道了以生色底物l -焦谷氨酰甘氨酰- L -精氨酸-对硝基苯胺盐酸盐为底物测定尿激酶效价的方法。
A method is reported for the determination of Urokinase potency using the chromogenic substrate L-pyroglutamyl-glycyl-L-arginine-p-nitroaniline hydrochloride.
方法以对氧磷为底物测定30例正常人、52单纯性脂肪肝患者、41例脂肪性肝炎患者、31例脂肪性肝硬化患者血清pon1活性。
Method Serum PON1 activity was measured by using paraoxon as substrate in 30 case of norm, 52 of simple fatty liver, 41 of steatohepatitis and 31 of fatty liver cirrhosis.
本文采用过硼酸钠作底物的高锰酸钾反滴定法测定了红细胞溶血制品及心肌匀浆中的过氧化氢酶活力。
The activity of catalase in hemolysates of erythrocytes and myocardial homogenate in rats was measured by back titration method with permanganate, using perborate as the substrate.
用l-酪氨酸作底物，测定了牛磺酸对酪氨酸酶的非竞争性抑制。
The noncompetitive inhibition of taurine on tyrosinase was determined with L - Tyrosine as substrate.
研究了以磷酸苯酯为底物微分脉冲伏安法测定碱性磷酸酯酶(ALP)的方法。
A new differential pulse voltammetric method for detection of alkaline phosphatase (ALP) using phenyl phosphate as substrate was developed.
目的以谷胱甘肽-S -转硫酶(GST)为模型，探讨有产物抑制时用积分法分析酶反应过程测定底物量的可靠性。
Objective To investigate the reliability of the integrated method for kinetic assay of substrate in the presence of product inhibition using glutathione-S-transferase (GST) as model.
方法：以生色底物法进行测定。
方法采用不同底物试餐测定空腹和试餐后呼气氢浓度动态变化，判断消化道功能状态。
Methods Judge digestive tract function by examining expiratory hydrogen concentration change during empty stomach and after eating different substrate.
为提高碱性磷酸酶（ALP）活性测定的灵敏度，对以4-甲基伞形酮磷酸酯（4-MUP）为底物的荧光法进行了研究。
In order to improve the sensitivity of detection of alkaline phosphatase(ALP)activity, a fluorometric method based on 4-methylumbelliferyl phosphate(4-MUP)as substrate was investigated.
研制出一种能快速测定水中有机磷农药的植物酯酶片和底物片。
Plant-esterase tablet and substrate tablet were developed for rapid detection of organophosphorous pesticides in water.
多酚氧化酶活性测定是用邻甲氧基苯酚为底物，用分光光度法测定。
Taken O-Methoxyphenol as the substrate, the polyphenol oxidase activity was determined by the spectrophotometry.
方法通过外源性底物磷酸化方法，测定了16例正常皮肤、22 例银屑病皮损、5 例皮肤鳞癌组织角质形成细胞膜酪氨酸蛋白激酶（ T PK）的活性。
Method The activity of tyrosine protein kinase(TPK) was determined by using exogenic substrates in 16 samples of normal skin, 22 psoriatic lesion and 5 skin squamous carcinoma.
方法建立豚鼠异种血清过敏性休克模型，采用专性底物对模型鼠的血清，肺，气管类胰蛋白酶活力进行测定。
Methods Sera and tissues were collected from anaphylactic shock guinea-pigs, and the enzyme activity was tested colormetrically using special substrate, BAPNA.
利用底物诱导呼吸法测定微生物生物量以及细菌、真菌生物量的变化表明，微生物在凋落叶分解过程中的演替遵循一定的路线。
The dynamics of microbial biomass and bacterial and fungal biomass measured from substrate-induced respiration (SIR) kept to some succession route during litter decay.
研究了对硝基磷酸苯酯（PNPP）为底物伏安法测定碱性磷酸酯酶（ALP）的方法。
A new electrochemical voltammetric enzyme immunoassay system was investigated based on p-nitrophenyl phosphate (PNPP)-alkaline phosphatase (ALP) coupling reaction.
应用发色底物法测定所有患者血浆PAI-1的活性，并分析其与NSTE-ACS患者近期预后及预后相关因素之间的关系。
We have a study on the relationship between PAI-1 and the recent prognosis of patients with NSTE-ACS and the conventional factors being significant to the recent prognosis of patients with NSTE-ACS.
用L—酪氨酸作底物，测定了银杏叶提取物对酪氨酸酶的非竞争性抑制作用。
The noncompetitive inhibition of EGb on tyrosinase was determined with L-Tryosine substrate.
还描述了鉴 别酶活性调节剂或新的酶底物的筛选测定法。
Screening assays to identify modulators of enzyme activity or new enzyme substrates are also described.
用猪油作为底物，测定了不同溶剂提取物的抗氧化性能。
Antioxidations from extracts of different solvents are determined by lard as substrate.
建立了紫外分光光度法测定青霉素酶对青霉素底物动力学参数的方法。
UV spectrophotometric method to estimate the kinetic parameters of penicillinase on penicillin substrates was established.
目的建立血管紧张素转化酶(ace)底物的合成方法，探讨血清ace活性的测定方法。
Objective To establish the synthetic method of the substrate for angiotensin-converting enzyme (ACE), and investigate the assay method of serum ACE.
以谷胱甘肽S转硫酶（GST）为模型，探讨有产物抑制时用积分法分析酶反应过程测定底物量的可靠性。
To investigate the reliability of the integrated method for kinetic assay of substrate in the presence of product inhibition using glutathioneStransferase(GST) as model.
方法：测定酶催化反应后底物浓度的变化，计算GST活力单位。
Methods:By detecting the concentration of the substrate after enzyme added the activity of the enzyme in the fluid was calculated.
方法采用多巴色素法，底物为L-多巴，测定山慈姑及其伪品提取物对酪氨酸酶的激活作用；
MethodsTyrosinase activity was determined by the dopachrome method using L-DOPA as the substrate and the amount of dopachrome in the reactiom mixture was measured by spectrophotometer.
在用于实际试样的回收率测定中，结果良好。(2)提出了一种基于酶催化沉积底物和生物素-亲和素两步放大的超灵敏免疫检测方法(第3章)。
An amplified immunoassay with improved sensitivity has been proposed based on two-step amplification procedures of enzyme-catalyzed precipitation and biotin-streptavidin binding system (in Chapter 3).
在用于实际试样的回收率测定中，结果良好。(2)提出了一种基于酶催化沉积底物和生物素-亲和素两步放大的超灵敏免疫检测方法(第3章)。
An amplified immunoassay with improved sensitivity has been proposed based on two-step amplification procedures of enzyme-catalyzed precipitation and biotin-streptavidin binding system (in Chapter 3).
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