应用聚合酶链反应得到2株蓝绿藻的毒素聚酮合成酶(PKS)基因，并进行基因序列分析。
Gene of polyketide synthase (PKS) from 2 toxin-producing cyanobacteria was prepared by polymerase chain reaction and the gene sequence was analyzed.
方法应用聚合酶链反应限制性片段长度多态性技术检测296例两个基因多态位点的等位基因、基因型。
MethodGenotypes and alleles of polymorphisms of both genes were determined with polymerase chain reactionrestriction fragment length polymorphism assay (PCRRFLP) of 296 subjects in Han Chinese.
方法：应用聚合酶链反应（PCR）-双酶切法，对23例CMT1患者和30例正常人进行基因特异性连接片段的检测。
METHODS:Polymerase chain reaction(PCR) combined with restriction enzyme digest ion were used to detect gene specific junction fragments of the 23 CMT1 patients and 30 normal controls.
方法应用聚合酶链反应限制性片段长度多态性分析方法(PCR -RFLP)对40例口腔鳞癌组织中apc基因的杂合缺失(LOH)进行检测。
Methods We used the polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP) to examine 40 oral squamous cell carcinomas for loss of heterozygosity (LOH) at APC.
方法在140名汉族健康人的外周血中，应用聚合酶链反应（PCR）限制性片段长度多态性分析（RFLP）及多重PCR技术，进行NAT1等位基因分型研究。
Methods Using multiple PCR and PCR-RFLP methods, we studied the NAT1 genotypes and its genetic polymorphisms of the peripheral blood samples from 140 Han people.
方法应用聚合酶链反应（ PCR）、DNA测序和限制性片段长度多态性（RFLP）等技术对1个帕金森病家系及120例散发性帕金森病患者进行PINK1基因R492X的突变分析。
Methods One family and 120 sporadic patients with Parkinson's disease were studied using polymerase chain reaction, DNA sequencing and restriction fragment length polymorphic (PCR-RFLP) techniques.
依据聚合酶链反应(PCR)和酶联免疫吸附检测(ELISA)的方法目前被应用于主要食源性致病菌的检测。
Polymerase chain reaction-based (PCR-based) and enzyme-linked immunoabsorbent assay (ELISA) methods are now applied in the detection of major food-borne pathogens.
应用一种缓慢但高度准确的试验，检测病毒的基因物质，叫即时逆转录-聚合酶链反应，或是rRT - P CR，全部做了评价。
All had been evaluated using a slower but highly accurate test called real-time reverse transcription-polymerase chain reaction, or rRT-PCR, which checks for the genetic material of the virus.
目的建立应用任意引物聚合酶链反应进行真菌菌型鉴定的方法。
Objective To establish the method to identify fungus using the arbitrarily primed polymerase chain reaction (APPCR).
近年来聚合酶链反应(PCR)已应用于口腔细菌的研究。
Polymerase chain reaction (PCR) extensively utilized for research about oral bacteria recently.
方法应用实时监测聚合酶链反应检测这三种受体基因在雌激素受体阳性和雌激素受体阴性乳腺癌细胞系中的表达。
Methods These three genes were quantified by real-time quantification polymerase chain reaction (PCR) in er positive and er negative breast cancer cell lines.
目的探讨荧光定量聚合酶链反应(FQ - PCR)在巨细胞病毒性肝炎中的应用价值。
Objective To investigate the fluorescence quantitative polymerase chain reaction (FQ-PCR) in the giant cell viral hepatitis in value.
应用反向间接血凝试验（RPHA）、酶免疫测定（EIA）和聚合酶链反应（PCR）测定了家禽血清、卵黄和牛乳清中HBV标志物及人HBV-DNA。
The HBV markers and HBV DNA in poultry sera, Yolk and bovine milk whey were detected by reversed passive hemagglutination assay (RPHA), enzyme immunoassay (EIA) and polymerase chain reaction (PCR).
目的：用荧光定量聚合酶链反应(FQ - PCR)测定丙型肝炎病毒在临床的应用。
Objective To investigate the clinical application of the Fluorescence quantitative PCR (FQ-PCR) in the detection of HCV infection.
方法应用逆转录-聚合酶链反应及免疫组化检测PIM3在小鼠眼部组织中的表达。
Methods Using reverse transcription-polymerase chain reaction (RT-PCR), the PIM3 mRNA expression in normal murine ocular tissues was defected.
目的探讨荧光定量聚合酶链反应（FQ-PCR）技术诊断结核病临床应用价值。
Objective To evaluate the clinical value of fluorescence quantitative PCR(FQ-PCR) technique in diagnosing tuberculosis.
目的探讨实时荧光定量聚合酶链反应(FQ - PCR)在检测外周血清及单核细胞中hcvRNA含量的临床应用价值。
Objective To investigate the clinical application of real time fluorescence quantitive Polymerase Chain Reaction (FQ-PCR) in detection of HCV RNA in serum and peripheral blood monocular cells (PBMC).
目的：探讨聚合酶链反应在沙眼诊断中的临床应用价值。
Objective: To investigate the clinical value of Polymerase Chain Reaction in the diagnosis of trachoma.
目的：应用内转录间隔区(its)通用引物建立一种较为快速、简便的检测和鉴定念珠菌的聚合酶链反应(PCR)方法。
Objective: To set up a rapid and simple PCR assay for Candida detection and identification by using internal tran scribed spacer (ITS) universal primers.
应用逆转录聚合酶链反应(RT - PCR)半定量分析大鼠脑放射性损伤后海马区在不同时间、不同剂量水平IL - 6基因转录的动态表达。
Semiquantitative analysis of IL-6 in the hippocampus was done at different time and dose level after whole-brain irradiation with reverse-transcription polymerase chain reaction (RT-PCR).
方法：应用半巢式聚合酶链反应技术检测113例孕产妇血清及其新生儿脐血的TTV—DNA。
Methods: TTV-DNA of 113 cases of parturients' blood serum and neonatal umbilical blood were detected by semi-nested polymerase chain reaction.
方法应用荧光定量聚合酶链反应(FQ - PCR)对尖锐湿疣高危人群分泌物标本进行HPV - DNA分型检测。
Methods the secretion specimen coming from the high risk patients with CA were examined with fluorescent quantitative polymerase chain reaction (FQ-PCR) for genotype HPV-DNA.
目的评价聚合酶链反应(PCR)分子灯塔法检测临床标本中结核分枝杆菌(结核菌)的应用价值。
Objective To evaluate the value of PCR molecular beacon assay. In detecting mycobacterium tuberculosis in clinical specimens.
方法：应用多聚合酶链反应技术(PCRT)，对10份遗传性卵巢癌组织中BRCA1基因内部的D17S855微卫星位点进行LOH检测。
Methods: Using the polymerase chain reaction technique (PCRT), LOHs in 10 samples of hereditary ovarian cancer at intragenic loci were detected.
方法从Z 10病毒感染的细胞提取总rna，将逆转录聚合酶链反应(RT PCR)扩增的产物纯化后克隆于t载体并进行序列测定，应用dnas TAR软件分析比较。
Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR products was cloned into t vector, sequenced and analyzed by using DNASTAR software.
方法应用嵌套式逆转录聚合酶链反应技术检测印记基因p57kip2、LIT1、TSSC3在人类卵母细胞及植入前胚胎的正常表达。
Methods Using nested reverse transcription-PCR to analyze the expression of P57KIP2, LIT1, TSSC3 in human oocytes and preimplantation embryos.
结果：32例阳性标本经过聚合酶链杂交结果与常规鉴定结果相符。结论：聚合酶链反应杂交梳法鉴定结核杆菌简便、快速、特异性强，有较高的临床应用推广价值。
Results The detective results of 32 positive samples with PCR hybridization combing is a quick, simple and highly specific method in the detection of TB, and may has some values in clinical diagnosis.
方法：建立DI大鼠模型，应用逆转录-聚合酶链反应(RT - PCR)方法检测DI及逼尿肌稳定(DS)大鼠逼尿肌组织IP3R亚型变化。
Methods: To set up the model of DI rats, the IP3R isoforms expressions of detrusor tissue in DI rats and ds rats were detected by RT-PCR technique.
方法：应用免疫组织化学和聚合酶链反应—单链构象多态性分析（PCR-SSCP）检测10例舌白斑和32例舌癌，并结合临床资料进行分析。
Methods:Immunohistochemistry and PCR-SSCP were used to detect the expression of P53 protein and p53 gene mutation in 32 cases of TSCC and 10 cases of tongue leukoplakia.
方法：应用逆转录-聚合酶链反应(RT - pcr)法检测73例急性白血病患者及23例正常人的WT 1及LRP基因的表达。
Methods: Expressions of WT1 and LRP genes were measured in 73 patients with acute leukemia and 23 normal controls by RT-PCR method.
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