目的探讨荧光定量聚合酶链反应（FQ-PCR）技术诊断结核病临床应用价值。
Objective To evaluate the clinical value of fluorescence quantitative PCR(FQ-PCR) technique in diagnosing tuberculosis.
方法：应用半巢式聚合酶链反应技术检测113例孕产妇血清及其新生儿脐血的TTV—DNA。
Methods: TTV-DNA of 113 cases of parturients' blood serum and neonatal umbilical blood were detected by semi-nested polymerase chain reaction.
方法在140名汉族健康人的外周血中，应用聚合酶链反应（PCR）限制性片段长度多态性分析（RFLP）及多重PCR技术，进行NAT1等位基因分型研究。
Methods Using multiple PCR and PCR-RFLP methods, we studied the NAT1 genotypes and its genetic polymorphisms of the peripheral blood samples from 140 Han people.
方法应用嵌套式逆转录聚合酶链反应技术检测印记基因p57kip2、LIT1、TSSC3在人类卵母细胞及植入前胚胎的正常表达。
Methods Using nested reverse transcription-PCR to analyze the expression of P57KIP2, LIT1, TSSC3 in human oocytes and preimplantation embryos.
方法应用聚合酶链反应限制性片段长度多态性技术检测296例两个基因多态位点的等位基因、基因型。
MethodGenotypes and alleles of polymorphisms of both genes were determined with polymerase chain reactionrestriction fragment length polymorphism assay (PCRRFLP) of 296 subjects in Han Chinese.
方法：应用多聚合酶链反应技术(PCRT)，对10份遗传性卵巢癌组织中BRCA1基因内部的D17S855微卫星位点进行LOH检测。
Methods: Using the polymerase chain reaction technique (PCRT), LOHs in 10 samples of hereditary ovarian cancer at intragenic loci were detected.
方法应用聚合酶链反应（ PCR）、DNA测序和限制性片段长度多态性（RFLP）等技术对1个帕金森病家系及120例散发性帕金森病患者进行PINK1基因R492X的突变分析。
Methods One family and 120 sporadic patients with Parkinson's disease were studied using polymerase chain reaction, DNA sequencing and restriction fragment length polymorphic (PCR-RFLP) techniques.
目的探讨聚合酶链反应(pcr)加反相杂交技术在细菌DNA检测中的应用。
Objective To develop a molecular biologic technique for detection of bacterial DNA in all bacteria with PCR and reverse hybridization.
目的探讨聚合酶链反应(pcr)加反相杂交技术在细菌DNA检测中的应用。
Objective To develop a molecular biologic technique for detection of bacterial DNA in all bacteria with PCR and reverse hybridization.
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