方法应用流式细胞术和细胞免疫荧光染色技术。
Methods It was studied by flow cytometry and immunofluorescence.
本文应用流式细胞术对胃癌细胞的DNA含量进行了分析。
Using flow cytometry the DNA content of gastric cancer cells Were analysed .
应用流式细胞术分析烟草细胞在交变应力作用下细胞周期的变化。
Flow cytometry was used to measure cell cycle parameters in tobacco cell suspensions acted by alternative stress.
方法：应用流式细胞术检测80例大肠癌标本中CD34的表达。
Methods: Flow cytometry (FCM) was used to detect the expression of CD34 in 80 cases cancer of colon.
应用流式细胞术对80例喉癌前病变细胞中的DNA含量进行定量检测。
The amount of DNA in precancerous cells with larynx were determined in 80 cases.
应用流式细胞术、DNA琼脂糖凝胶电泳、透射电镜等多种手段检测心肌细胞凋亡的改变。
The myocardial cells of each groups were taken for detecting apoptosis by the flow cytometry and DNA gel electro-phoresis.
目的：应用流式细胞术，建立一种准确、外周血嗜酸性细胞及其相关分子的快速测定方法。
AIM: to set up an accurate and rapid method to detect the phenotype of peripheral blood eosinophils using flow cytometry.
方法应用流式细胞术分析人胃癌MGC803细胞在DADS处理前后细胞周期分布的变化。
Methods cell cycle of MGC803 cell treated with DADS in vitro was analyzed by flow cytometry.
目的探讨应用流式细胞术和活细胞荧光染料CFSE检测T淋巴细胞各亚群增殖反应的方法学。
Objective To evaluate the method of determining the proliferation of human T lymphocyte subpopulations by using flow cytometry and carboxyfluorescein diacetate succinimidyl ester(CFSE) labeling assay.
方法应用流式细胞术检测16例正常人和32例肾癌患者外周血淋巴细胞CD25、Ki67的表达。
Methods Peripheral blood lymphocytes CD25 and Ki67 were detected by flowcytometry in 32 patients with RCC and 16 healthy individuals served as controls.
应用流式细胞术进行DNA倍体分析和TUNEL分析，检测两药单用与联用对细胞周期及早期凋亡的影响；
The fluorescence flow cytometry (FCM) DNA assay and TUNEL were applied to detect cell cycle phase and cell apoptosis. the endogenous VEGF contents were detected by ELISA method.
方法取新鲜NSCLC标本28例，应用流式细胞术测定CD44，同时测定凋亡细胞峰，并与癌旁肺组织进行比较。
Methods With flow cytometry, expression of CD44 and apoptosis cell peak were detected in 28 fresh specimens of NSCLC and normal pulmonary specimens.
方法应用流式细胞术检测了35例急性淋巴细胞性白血病患者骨髓细胞DNA含量(DI)、倍体和细胞增殖活性(PI)。
Methods The DNA index (DI) and proliferation index (PI) in aspirated myeloid cells were determinated in 35 patients with ALL by flow cytometry (FCM).
应用流式细胞术对5例先天性中胚层肾瘤、96例肾母细胞瘤和27例正常肾石蜡包埋组织进行了DNA含量和增殖活性分析。
The DNA content and cell cycle in paraffin-embedded sections collected from 5 cases of congenital mesoblastic nephroma. 96 cases of nephroblastoma and 27 normal kidneys were studied by flow cytometry.
方法应用流式细胞术测定184例消化道恶性肿瘤患者癌细胞DNA含量，并分析DNA倍体和胃肠道恶性肿瘤生物学行为关系。
Methods DNA ploidy of 184 cases with malignant cancer of the gastrointestinal tract was measured by flow cytometry. The DNA ploidy and cell cycle distribution were analyzed.
目的应用流式细胞术(FCM)测定胸液细胞DNA倍体，并同时检测胸液癌胚抗原(CEA)水平，以探讨其在恶性胸液诊断中的价值。
Objectives to investigate the diagnostic value of DNA analysis and combined with Carcinoembryonic antigen (CEA) for malignant pleural effusions by FlowCytometry (FCM).
方法应用MTT法检测不同浓度的5-氮-2’-脱氧胞苷对胆管癌细胞株QBC939存活率的影响，应用流式细胞术检测5-氮-2’-脱氧胞苷对QBC939细胞生长周期及凋亡率的影响。
Methods The survival rate and apoptosis rate of QBC939 after being treated with different density of 5-aza-2'-deoxycytidine were detected by using the method of MTT and flow cytometry.
本文就流式细胞术在微生物学中的应用作一综述。
This article reviews the application of FCM in the microbiology in detail.
流式细胞术已成为诊断血液系统恶性肿瘤不可缺少的工具，广泛应用于白血病的临床检测。
Recently, flow cytometry becomes one of the obbligato tools in the procedure of diagnosis of hematological malignant tumors and has been widely used in clinical test.
应用四色荧光活化流式细胞术(FACS)检测CD 34 +细胞其粘附分子及趋化因子受体CXCR -4的表达谱。
Four color fluorescence activated cell sorting (FACS) analysis was applied to detect the expression profiles of adhesion molecules and chemokine receptor CXCR 4 on CD34 bright cells.
方法：应用基因芯片、反转录PCR（RT-PCR）、流式细胞术等方法从分子和蛋白水平检测两种细胞系中CXCR4的表达情况。
Methods: Gene chip and RT-PCR were used to study expression of CXCR4 mRNA in two cell lines and the CXCR4 surface expression was also examined by flow cytometry analysis.
方法应用免疫细胞化学和流式细胞术方法。
Methods The immunocytochemical and flow cytometry were used.
流式细胞术是近年来应用较广泛的一种现代细胞分析技术。
Flow cytometry is a modern cell analysis technology widely used recently.
应用氯化镉致人胚肺成纤维细胞恶性转化实验、染色体分析、流式细胞术对氯化镉的致癌机理进行研究。
The mechanism of carcinogenesis induced by CdCl 2 was studied through experiments of the malignant transformation of human embryo lung fibroblast in vitro, chromosome analysis and flow cytometry.
方法：四氮唑蓝比色法(MTT法)观察梁金菇多糖对HL 6 0细胞的抑制率，应用形态学、流式细胞术、DNA凝胶电泳等方法检测细胞凋亡的发生。
METHODS: The proliferation inhibition effects of LJPS on HL-60 cells was observed by MTT colorimetry. Apoptosis was detected by morphology, flow cytometry and DNA agarose gel electrophoresis.
方法：应用形态学观察，MTT分析法，DNA梯状电泳，流式细胞术检测术，研究紫杉醇对成骨肉瘤细胞系SO SP- 96 0 7的细胞毒效应。
Methods: The cytotoxic effect of paclitaxel on human osteosarcoma cell line SOSP-9607 was examined by MTT assay, morphological observation, gel electrophoresis of DNA, and cell morphology.
方法：应用形态学观察，MTT分析法，DNA梯状电泳，流式细胞术检测术，研究紫杉醇对成骨肉瘤细胞系SO SP- 96 0 7的细胞毒效应。
Methods: The cytotoxic effect of paclitaxel on human osteosarcoma cell line SOSP-9607 was examined by MTT assay, morphological observation, gel electrophoresis of DNA, and cell morphology.
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