方法应用PCR及单链构象多态性(SSCP)分析技术结合基因序列测定方法确定突变类型。
Single strand conformation polymorphism (SSCP) essay and sequence analysis of the PCR product were used to ascertain the gene mutation.
方法从Z 10株病毒感染的细胞提取总rna，将逆转录pcr扩增的产物纯化后克隆于t载体并进行序列测定，应用dnastar软件分析比较。
Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR product was cloned into t vector, sequenced and analyzed using DNASTAR software.
方法从Z 10病毒感染的细胞提取总rna，将逆转录聚合酶链反应(RT PCR)扩增的产物纯化后克隆于t载体并进行序列测定，应用dnas TAR软件分析比较。
Methods the total RNA were prepared from Z10 virus infected cells and the RT PCR products was cloned into t vector, sequenced and analyzed by using DNASTAR software.
基因序列测定和分析结果进一步表明该毒素基因高度保守。
PCR and sequence analysis were used to detect the toxin gene.
方法：通过菌落特征、显微形态、ITS序列测定及其系统发育分析对菌株WC1016进行鉴定。
Methods:The strain of WC1016 was identified by culture characteristics, morphological characteristics, ITS sequence and phylogenetic analysis.
对BDV阳性产物进行基因序列测定、同源性和氨基酸顺序分析，绘制系统发生树，初探bdv感染的分子流行病学特征。
Further, the gene sequence and amino acid sequence for BDV positive product were analyzed to establish the molecular epidemiologic characteristic by drawing phylogenetic tress.
其中碳末端45 0个核苷酸的序列测定和分析提示，该7株病毒为麻疹野病毒H1基因型。
Sequence analysis of 450bp of C-terminus of the N gene indicated that all of the 7 isolates were H1 genotype.
PCR产物经限制性内切酶消化、序列测定及分析。
方法设计出针对各片段的特异性引物，用P CR方法从Z 37病毒感染的细胞提取细胞总rna，逆转录扩增、产物克隆t载体，纯化后测序，测定的序列应用DNASTAR软件比较分析。
Methods the total RNA was extracted from Z37 virus infected cells and the RT-PCR products were cloned into t vector, sequenced and analyzed by DNASTAR software.
EBI的下的蛋白质组分析数据库，提供已经测定了全序列基因组的器官的已经预测过结构的蛋白质组的统计和比较分析。
The proteome Analysis Database at EBI provides statistical and comparative analyses of the predicted proteomes of organisms for which there are fully-sequenced genomes.
方法利用聚合酶链反应（PCR）产物直接测序，对10例血液标本进行了HLA-DPB1、DRB外显子2的序列分析，并将测定结果与基因型核酸序列数据库进行比较。
Methods Sequences of HLA-DPB1, DRB exon2 of 10 samples were analysed by direct sequencing of PCR products. The results of sequencing were compared with their corresponding sequence data.
对分离到的HEV71阳性分离株进行VP1编码区基因扩增，核苷酸序列测定和同源进化分析。
And identified HEV71 isolates were performed by gene amplification of VP1 coding region, nucleotide sequencing and homology analysis of evolution.
它们可以被广泛地应用于DNA序列测定、新基因的检测和突变基因的分析。
Gene chips can be widely used for sequencing of DNA, and for detecting new genes and mutations.
并对发现突变的基因进行分析。方法应用PCR扩增、DNA序列测定等技术分析所有AR基因外显子及其邻近DNA序列片段；
Methods PCR and DNA sequencing were performed to study the AR gene mutation; Mbo I restriction endonuclease was used to detect existence of the mutation in normal controls;
并对发现突变的基因进行分析。方法应用PCR扩增、DNA序列测定等技术分析所有AR基因外显子及其邻近DNA序列片段；
Methods PCR and DNA sequencing were performed to study the AR gene mutation; Mbo I restriction endonuclease was used to detect existence of the mutation in normal controls;
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