方法：利用植物细胞平板技术培养。
方法采用念珠菌显色平板培养法鉴定，用念珠菌药敏测定试剂盒进行药敏试验。
Methods Candida color culture medium was employed for identification of the fungus and drug sensitive test was performed by fungal identification strip.
方法用涂布平板法对水中可培养菌数进行计数。
Plate count method was used to count the culturable cells in three different water samples.
方法：富集培养、血平板分离、摇瓶培养和排油圈测定。
Method: Based on enrichment culture, blood plate separation, the flask culture and exclusive circle of methods such as oil.
方法：采用鸡蛋斜面培养基，亚碲酸钾血琼脂平板和肉汤培养基观察其生长特性、测定多种生化反应性、毒力和药物敏感性。
Methods: To observe the cultural characteristics with the medium of inclined plane of egg, AGAR plate with blood and potassium tellurite, and broth.
方法蜜环菌菌株生物学特性的观察采用平板培养法，菌丝体多糖含量的测定采用比色法。
METHODS Plant cultivation was used to observe the biological characteristics of Armillaria mellea. The contents of hypha polysaccharide were determined by using colorimetric method.
方法用含芦荟提取物的平板培养供试菌株，确定最小抑菌浓度；采用滤纸片法研究了芦会提取物与蔗糖、食盐的协同抑菌性能。
Methods The minimal inhibitory concentration was determined by cultivating them in culture medium containing extracts, and the synergistic effect was studied by paper filtering method.
稳定剂为琥珀酸钠，再生培养方法为平板双层法。原生质体形成率和再生率分别达到93.8%和37.5%。
The rates of formation and regeneration of protoplasts of strain S23 had reached to 93.8% and 37.5% respectively.
稳定剂为琥珀酸钠，再生培养方法为平板双层法。原生质体形成率和再生率分别达到93.8%和37.5%。
The rates of formation and regeneration of protoplasts of strain S23 had reached to 93.8% and 37.5% respectively.
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