目的：研究不同分泌信号对豹蛙酶（ONC）在巴斯德毕赤酵母中分泌效率的影响。
Objective:To investigate the effects of different secretion signals on efficiency of onconase(ONC) secretion in Pichia pastoris expression system.
融合蛋白基因在巴斯德毕赤酵母中进行表达后通过阳离子交换层析、反向层析和凝胶过滤对表达产物进行了分离纯化。
HSA-AX15(R13K ) fusion protein was purified to homogeneity by cation exchange chromatography, re verse phase chromatography and gel filtration after expressed in pichia pastor is.
为了获得大量有活性的重组蛋白酶便于今后的工业应用，我们将蛋白酶WF146克隆到分泌性表达宿主枯草芽胞杆菌和巴斯德毕赤酵母中。
For the convenience of production and purification, Bacillus subtilis and Pichia pastor is were used as hosts for the expression of protease WF146.
目的：利用巴斯德毕赤酵母表达系统获得重组的人层黏连蛋白LG1-3组件蛋白，为进一步研究LG1-3的结构和功能间的关系奠定基础。
Objective: To express the LG1-3 module of human laminin alpha 4 chain in P. pastoris expression system for studying the function of LG1-3 module.
目的：利用巴斯德毕赤酵母表达系统获得重组的人层黏连蛋白LG1-3组件蛋白，为进一步研究LG1-3的结构和功能间的关系奠定基础。
Objective: To express the LG1-3 module of human laminin alpha 4 chain in P. pastoris expression system for studying the function of LG1-3 module.
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