获得了很多差异片段的克隆,并从其中选择3个进行测序。
Gain many clone of gene fragment on differential expression, choice 3 of them for sequencing, and blast the sequence in Genebank.
目的筛选和分析胃癌和正常胃组织间DNA甲基化差异片段。
Objective: To isolate and analyze the differentially methylated DNA sequences between gastric cancer and normal gastric mucosa.
随机挑取约200个阳性克隆进行差异筛选。共获得29个差异片段。
About 200 positive clones selected randomly were differentially screened, and 29 differentially expressed genes were obtained.
目的:筛选胃癌和癌旁正常胃组织间DNA甲基化差异片段并进行相关生物信息学分析。
Objective To isolate the differentially methylated DNA sequences betweengastric cancer and adjacent normal gastric mucosa and analyze them with relative bioinformatics methods.
主要结果如下:1。通过骏枣抗病和感病品系的AFLP分析,获得了11条差异片段。
The main results obtained were as following: 1 11 differential expression bands were screened by AFLP among the resistant and sensitive strains in Junzao.
您需要将模型片段的新版本与以前的版本相比较以查看两者之间的差异。
You need to compare the new version of the model fragment with the previous version to see the difference.
在不同的数据库中,访问这些XML片段的方式可能有所差异。
The ways to access these XML fragments might differ between databases.
虽然有一些小差异,但是这个片段看起来应该很熟悉。
The snippet should look familiar, albeit with small differences.
清单2和 清单3 中的代码片段说明了与标准Apache配置的基本差异。
The snippets in Listings 2 and 3 illustrate the essential configuration points that vary from a standard Apache configuration.
这个小组以相隔了13代的两个男性为测试对象,在其Y染色体相同区域上选取长约10, 000, 000核苷酸片段进行了测序,并计算了两者之间的差异。
The team sequenced the same piece of DNA - 10,000,000 or so letters or 'nucleotides' from the Y chromosome - from two men separated by 13 generations, and counted the number of differences.
现在,我们终于开始着手确定非编码DNA(不产生蛋白质的分子片段,习惯上被认为是“垃圾”)的差异性对人类的影响水平。
Now we are finally pinning down how much differences in non-coding DNA - stretches of the molecule that don't produce proteins and used to be considered "junk" - shape who we are.
目的:探讨血清细胞角蛋白19片段(CYFRA21 - 1)在不同民族食管癌患者中的差异。
Objective to investigate the serum levels of cytokeratin 19 fragment (CYFRA21-1) in Chinese people of different nationalities with esophageal cancer.
这两个差异是,在再现模式粘贴的片段没有覆盖背景,但是嵌入到背景使该片段的某些部分是可见的。
The difference between the two is that in Emersion Mode the pasted fragment does not overlay the background, but embeds into the background so that only certain parts of the fragment are visible.
结论:获得的差异表达片段为进一步克隆甲状腺癌相关基因及功能研究奠定了良好的基础。
Conclusions: the differentially expressed fragments establish a favorable foundation for cloning genes related to thyroid carcinoma and continuing with their functional studies.
差异表达的片段共66条,其中46条来自子黄绿色柿果,20条来自于绿。
Finally, there were 66 differential fragments chosen from, 20 of them belonged to green fruit and 46 belonged to turning yellow fruit.
结果:从正常肝组织与模型组肝癌组织中分离出32个差异显示的基因片段;
The results showed that 32 genetic segments indicating difference were separated from the normal liver tissues and the liver cancer tissues of the model groups.
不同引物所扩增出的条带数目片段大小不同,同一条引物在不同晶系之间也有较大差异。
Three primers were screened and all of them were polymorphic, the band numbers and the fragment length were different in each primer.
通过对先期实验已证实存在BDV感染的人体标本和来源于感染动物的病毒标准品进行检测,拟了解感染人和动物的该病毒片段是否存在差异。
The research is for understanding whether or not there is any difference in the fragment between from animal and human beings infected with BDV proved by previous research.
结果表明:三个品种鸡的心肌、肝脏组织分别检测到18和20条差异表达的基因片段。
Among the three breeds, 18 and 20 differential expressed fragments were detected in cardiac muscle and liver tissues, respectively.
应用该优化的方法,比较了H和SP的基因表达图谱,分离了13个差异表达的片段。
Using this improved method, we analyzed the gene expression patterns of H and SP, achieving 13 differentially expressed cDNA fragments.
利用RAPD技术对TBT处理细胞提取的DNA进行分析,在各TBT处理组与对照组相比共扩增出72条差异性片段。
There were 72 differential DNA fragments in the cells disposed to different levels of TBT compared with control by the random amplified polymorphic DNA (RAPD).
用改良技术研究热适应大鼠下丘脑基因的差异表达,发现了一个差异表达基因片段,斑点杂交证实为阳性片段。
A differentially expressed gene was identified from hypothalamus of heat acclimated rats using the improved technique, which is verified by dot blot.
其中差异表达片段C15、A45、D22、D73和E94在植物与害虫相互作用相关分子机制研究中首次被发现。
The fragments of C15, A45, D22, D73 and E94 were firstly found in the studies of molecular mechanism of plant-insect interaction, up to day.
并从中筛选出了53个EST片段对其进行了序列分析和同源性检索,其中28个EST片段和报道的基因有100%同源性,有25个EST片段和报道过的基因有差异但高度同源。
We picked out 53 ESTs to make sequence analysis and homology blast and found that 28 ESTs was 100% homologous and 25 ESTs was different but high homologous to reported genes.
结论这2条差异显示新基因片段很可能是与大肠癌相关的致癌基因。
Conclusion These 2 specifically expressed cDNA fragments isolated by DD-PCR might be the colorectal carcinoma-related oncogenes in quest.
目的分离晕船适应大鼠下丘脑差异表达基因片段,以探讨晕船适应机理。
Objective To construct subtracted cDNA library in hypothalamus of the seasickness adaptive rats for providing theoretical basis for effective adaptive training against seasickness.
采用病例对照研究的方法,用聚合酶链反应(PCR)和限制性片段长度多态性分析(RFLP)研究TS患者与健康对照间DRD2P、COMT基因的基因型频率有无统计学差异。
DRD2P and COMT genotyping were carried out using PCR followed by RFLP and statistical analysis of genotype frequencies between ts patients and healthy controls was performed using SPSS programme.
对两个抗病品种表达量上调的片段进行回收,总共得到了13条差异序列,其中有6条差异序列在两个品种中均有表达。
The up-regulated bands of the two resistance varieties were picked out and cloned, 13 TDFs were gained in all, and 6 TDFs are both in the two varieties.
对两个抗病品种表达量上调的片段进行回收,总共得到了13条差异序列,其中有6条差异序列在两个品种中均有表达。
The up-regulated bands of the two resistance varieties were picked out and cloned, 13 TDFs were gained in all, and 6 TDFs are both in the two varieties.
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