方法：应用DE52离子层析法。
采用柱层析法分离纯化番茄红素。
Separation and purification of lycopene by column chromatogram were studied.
抗体采用蛋白a和肽亲和层析法纯化。
Antibodies are purified by protein a and peptide affinity chromatography.
方法：薄层层析法及大孔树脂分离方法。
Method: Thin layer chromatography and macroporous resin separation method were used.
方法采用硅胶、聚酰胺和ODS柱层析法。
METHODS Silica, polyamide and ODS column chromatography were used.
方法运用重结晶和柱层析法进行分离和纯化。
METHODS Recrystallization and column chromatography were used to get purer hyodeoxycholic acid and some other components.
纸型沉淀层析法的特点是：简便，快速而又灵敏。
Precipitation Chromatography on paper is sensitive, fast and simple in manipulation.
用硅胶柱层析法分离纯化，用光谱法进行结构鉴定。
METHODS ? The isolation and purification was performed by silica gel column chromatography and the structures were identified by spectral analysis.
采用薄层层析法对不同沸点的有机化合物进行了测定。
Organic compounds with various boiling points are determined by thin layer chromatography.
采用薄层层析法（TLC）分离菊芋汁中的低聚糖成分。
Thin layer chromatography(TLC) was applied to the separation of oligosaccharides in juice of Jerusalem Artichoke.
目的探讨免疫层析法检测结核抗体在肺结核诊断中的应用价值。
Objective to investigate the application value of immunochromatography for the detection of anti-tuberculosis antibody in diagnosing pulmonary tuberculosis.
方法：采用免疫层析法和ELISA法检测血清中特异性抗体。
Methods; the specific antibody in the serum were detected by immunochromatography and ELISA.
研究了应用金属螯合亲和层析法纯化重组类人胶原蛋白的条件。
Recombinant human-like collagen with a hexahistidine tail attacked to the carboxyl end could be easily purified by Metal Chelated Affinity Chromatography (MCAC).
只有当有机化合物的沸点较高时才有可能用薄层层析法进行测定。
Only when the boiling point of organic compounds is high enough, they can be determined by thin layer chromatography.
研究了离子沉淀法和大孔吸附树脂层析法结合制备茶多酚的工艺。
Combining the ion precipitation with column chromatography to prepare tea polyphenol was studied.
方法：采用系统溶剂法提取，硅胶干柱层析法进行化学成分分离。
Method: Systematic solvent method is used to extract and silica gel dry column sheet gradation was used to separate the chemical composition.
方法：采用了溶媒分离法结合柱层析法、薄层层析法进行成分分离。
Methods: to isolate the alkaloids, the solvent-extraction together with the column, chromatography and thin-layer chromatography methods were used.
用层析法检验DBC-偶氮氯膦，纯产品只有一个单一的蓝色斑点。
The purity of DBC-chlorophosphonazo was checked by chromatography and a single blue band was obtained for the pure product.
精制方法主要包括专一性溶剂法、酸碱法、酶水解法和柱层析法等。
Specific solvent separation, acid and alkali treatment, enzymatic hydrolysis and column chromatography are the main methods to be used in the refinement of curcumin.
采用硫酸铵沉淀法和阴离子交换层析法从鸡蛋清内提取高纯度卵铁传递蛋白。
High purity ovotransferrin was extracted from albumen by precipitation with ammonium sulfate and ion exchange chromatography in the study.
方法：采用薄层层析法，对样品中丹参、木香、葛根和三七进行了鉴别。
METHODS:TLCS method was used to identify Radix salviae miltiorrhizae, Radix aucklandiae, Radix puerariae and Radix notoginseng.
介绍了一种快速、简便、有效的短柱层析法，并设计一套易于推广的实验装置。
This paper presents the novel kind of fast, simple and effective chromatography of the short column, and designs the new set of easily spreading device.
方法用不同的展开剂，采用薄层色谱法和纸层析法对全蝎抗凝活性成分进行鉴定和分析。
Methods Under the various separating condition, the anticoagulating active components of scorpion were appraised with thin layer chromatography(TLC) and paper chromatography.
结果用改良的薄层层析法分析多糖的单糖组成，得到清晰的图谱，与气相色谱法结果一致。
Results Distinct figures indicating the monosaccharide composition of polysaccharides obtained by the modified TLC were well consistent with those from GC method.
采用DEAE—纤维素离子交换柱层析法对红枣多糖进行组分分析，可得到五种多糖组分。
Using DEAE-Cellulose to separate the polysaccharides from jujube Mill, five content has been acquired.
利用不同溶剂提取叶绿体色素，再用纸层析法对叶绿体色素进行分离，并对分离效果进行比较。
Different solvents were used to distill chloroplast pigment, and then the extractive was separated with paper chromatography.
确立了以有机溶剂提取海藻中的生长素，以乙醚萃取法和柱层析法对生长素进行分离纯化的方法。
The method of extracting the auxin in seaweeds with organic solvent, separating and purifying the auxin by ether extraction method and column chromatography has been established.
实验结果表明，等电点沉淀、凝胶柱层析法和阴离子交换法适合于分离水牛乳中主要过敏原成分。
The results showed that the methods of isoelectric precipitation, gel-filtration chromatography and anion-exchange chromatography were suitable for purifying the major allergens in buffalo milk.
结论：通过两步层析法分离纯化得到ABU- LAO，它能诱导小鼠发生严重的急性肺损伤。
CONCLUSION: ABU-LAO was (purified) by a two-step chromatographic process. The enzyme can induce serious acute lung injury when being injected into mice.
IPTG诱导表达融合蛋白，包涵体蛋白经复性后亲和层析法纯化，ELISA方法测定蛋白活性。
Fusion protein expression was induced by IPTG. After renaturation, the protein was purified by affinity chromatography and the bioactivity was examined by ELISA.
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