目的探讨建立有效的小鼠胚胎成纤维细胞饲养层，用于胚胎干细胞的培养。
Objective To study the isolation and culture and application of Kunming mouse embryonic fibroblasts(MEF).
目的：建立ICR小鼠胚胎成纤维细胞饲养层培养体系，用于胚胎干细胞建系及研究。
Objective: To establish feeder layer culture system of ICR mouse embryonic fibroblast (MEF) cells for isolation and culturing of embryonic stem (es) cells.
目的在本实验室建立小鼠胚胎成纤维细胞培养方法，并在此基础上用培养的细胞检测氯化镉、甲醛等外来化合物的细胞毒性反应。
Objectives: To set up the methods of mouse embryo fibroblasts culture in vitro and to evaluate the toxicity of xenobiotics such as chloride cadmium and formaldehyde by using these methods.
目的在本实验室建立小鼠胚胎成纤维细胞培养方法，并在此基础上用培养的细胞检测氯化镉、甲醛等外来化合物的细胞毒性反应。
Objectives: To set up the methods of mouse embryo fibroblasts culture in vitro and to evaluate the toxicity of xenobiotics such as chloride cadmium and formaldehyde by using these methods.
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