方法:(1)建立与改良小鼠成骨细胞的原代培养方法,并通过碱性磷酸酶和钙结节染色方法进行细胞来源鉴定。
Methods: (1) We built and improved the method of osteoblasts primary culture in vitro in mice, and identified the origination of osteoblasts by AKP and calcium nodes dyeing.
为了更好的了解胰岛素对于骨骼的影响,两个研究小组对实验小鼠进行了改造,使得它们的成骨细胞中不再有胰岛素受体。
To better comprehend insulin's effects on bone, both research teams engineered mice so that their osteoblasts no longer displayed insulin receptors.
目的:系统的研究小鼠胚胎干细胞的提取、体外培养扩增和向成骨细胞定向诱导分化。
Objective: research systematically into the isolation, culture and direct differentiation to osteoblast of embryonic stem cells.
细胞水平上淫羊藿乙酸乙酯萃取部位可以促进小鼠骨髓基质细胞向成骨细胞分化而不抑制其向脂肪细胞分化。
On cellular level, the fraction of ethyl acetate could promote osteogenic differentiation and showed no inhibition on adipogenic differentiation of MSCs.
结论:EGFR信号转导系统在在小鼠松质骨的形成过程中起着重要作用,其机制可能与调节成骨细胞向中心生长板的募集有关。
Conclusion EGFR signaling play an important role in trabecular bone formation, the partial mechanisms for which might be related to the regulation of osteoblast recruitment.
结论:EGFR信号转导系统在在小鼠松质骨的形成过程中起着重要作用,其机制可能与调节成骨细胞向中心生长板的募集有关。
Conclusion EGFR signaling play an important role in trabecular bone formation, the partial mechanisms for which might be related to the regulation of osteoblast recruitment.
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