目的探索巯基乙酸对非洲蟾蜍和小鼠卵母细胞体外成熟的影响。
Objective To investigate the effect of thioglycolic acid on oocyte maturation process in vitro in mice and Xenopus .
目的初步探讨高尔基体在小鼠卵母细胞体外发育进程中的作用。
Objective The aim of this study is to investigate the function of Golgi apparatus during in vitro development of mouse oocytes.
前言：目的探讨小鼠巨细胞病毒对小鼠卵母细胞体外成熟的影响。
Objective: to study the effect of murine cytomegalovirus on the in vitro maturation of mice immature oocytes.
目的探讨新的小鼠卵母细胞透明带膨胀（ZPD）辅助去核法的可靠性。
Objective: To study the reliability of the new enucleation method for mouse oocyte: zona pellucida dilating (ZPD) assisted enucleation method.
电场导入1,4,5 -三磷酸肌醇(IP_3)激活小鼠卵母细胞的研究。
The Study of Activation of Mouse Oocytes by Electrical Inducement of Inositol 1, 4, 5-trisphosphate (IP_3).
目的：探讨卵丘细胞在玻璃化冷冻中对小鼠卵母细胞发育潜能及细胞骨架的影响。
Objective: Study the effects of cumulus cells on the developmental potential and cytoskeleton of mouse vitrified-thawed oocytes.
蛋白合成抑制剂亚胺环己酮可以诱导小鼠卵母细胞发生孤雌活化，但其机制尚未完全阐明。
Cycloheximide(CHX), a protein synthesis inhibitor, can activate mouse eggs but the underlying mechanism is not fully known.
探讨细胞松弛素b和解冻程序对玻璃微细管法(GMP)玻璃化冷冻小鼠卵母细胞的影响。
Effects of cytochalasin B and thawing program on glass micropipette (GMP) vitrification of mouse mature oocytes were investigated.
改进的小鼠表面张力辅助去核法是一种适用于小鼠卵母细胞操作的快速、简单、实用的去核方法。
Modified surface tension assisted method is a efficient and simple enucleation method with the advantage of saving manipulating time and non-damage to mouse oocyte.
但是，在小鼠卵母细胞体外成熟过程中，热应激对卵母细胞以及随后孤雌胚胎发育能力的研究较少。
But there is little information available on in vitro parthenogenetic development of mouse oocytes that were exposed to heat stress during in vitro maturation.
结果表明，猪卵丘细胞能促进小鼠卵母细胞的体外成熟，但并不影响小鼠卵母细胞的受精和胚胎发育。
The results showed that porcine cumulus cells could enhance maturation of mouse oocytes in vitro but not affect in vitro the fertilization of the oocytes and subsequent embryo development.
方法采用小鼠卵母细胞体外培养、体外受精的方法研究了硫酸锰对小鼠卵母细胞成熟和受精能力的影响。
Methods The effect of manganese vitriol on meiotic maturation and fertilization ability of mouse oocyte was studied by culture of mouse oocyte and in vitro fertilization (IVF).
方法：采用小鼠卵母细胞体外培养、体外受精的方法研究了铅对小鼠卵母细胞的成熟和体外受精的影响。
Methods the effects of TGA on oocyte maturation and in vitro fertilization (IVF) in mice were studied by the method in vitro culture and IVF in mice oocyte.
方法：通过HIO与CVM分别获得100个、80个成熟小鼠卵母细胞，制备染色体标本进行核型分析。
Methods: 100 matured mouse oocytes were obtained by HIO and 80 by CVM. Their chromosomal complements were prepared and analyzed.
综合上述结果可以看出，6 -DMAP对小鼠卵母细胞的激活作用时间窗口具有卵龄依赖性但与起始刺激后的时间关系不大。
From the above results, we concluded that the temporal window for action of 6-dmap on the oocytes depended on the egg age of oocytes but independent of the starting time of the treatment with ethanol.
人卵冻贮：在成功玻璃化冷冻小鼠卵母细胞的基础上，再随机选用人卵母细胞，其冷冻方法、复温方法及统计学处理同动物实验。
Human experiment: Human oocytes were collected from our center, the cryopreserved method, rewarming method and statistical analysis method is same to animal experiment.
目的：研究EDS - 40玻璃化溶液冻贮小鼠及人卵母细胞的效果。
Objective: Study on the cryoprotective effect of the EDS-40 vitrification solutions to mice and human oocytes.
前言：目的以小鼠为研究模型，探索观察成熟卵母细胞纺缍体和染色体的方法。
Objective: This study is designed to find an effective method of observing the morphology of meiotic spindle and chromosome in mature oocytes, taking the mouse as a model.
发现小鼠成熟卵母细胞纺缍体和染色体的正常率分别为82%和86%。
The normal rates of meiotic spindle and chromosome were 82% and 86% respectively.
本文结合我们近些年对小鼠胚胎卵巢卵泡发育研究结果简要综述当前卵母细胞体外培养体系的研究进展。
We will focus on the advances in the oocyte culture models combining with our findings on the mouse fetal ovarian oocyte development in vitro in this text.
结果记录排卵前卵母细胞至扩展囊胚的小鼠早期胚发育全过程，制成微分干涉差显微图谱。
Results a DIC atlas of preimplantation embryos in full developmental stages was formed from preovulatory oocyte to expanded blastocyst.
模型组小鼠卵巢中颗粒细胞、卵母细胞的凋亡率与对照组比较，具有显著性差异（P<0.05）。
The differences were significant as compared with the low, middle and high dosage ZGP and control group(P<0.05).
模型组小鼠卵巢中颗粒细胞、卵母细胞的凋亡率与对照组比较，具有显著性差异（P<0.05）。
The differences were significant as compared with the low, middle and high dosage ZGP and control group(P<0.05).
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