目的：观察模拟咬合压力对人牙周膜成纤维细胞环氧化酶2 (COX 2)基因表达的影响。
Objective: To investigate the influence of simulated occlusion pressure on the cyclooxygenase-2 (COX-2) gene expression in human periodontal ligament fibroblasts (HPLFs).
目的：观察中间普氏菌和脆弱类杆菌的内毒素对人牙周膜细胞增殖和超微结构的影响。
AIM: to examine the effect of endotoxin of prevotella intermedia and bacteroides fragilis on proliferation and ultrastructure of periodontal ligament cells in vitro.
方法：通过原代和传代培养，获得性状稳定的人牙周膜细胞，使用动态机械应变细胞加载仪对细胞进行1%、10%和20%动态牵张应变加载。
METHODS: Primary human periodontal ligament cells were obtained and passaged. Then the cells were stretched by dynamic mechanical strain unit with 1%, 10% and 20% strain at different time.
方法：通过原代和传代培养，获得性状稳定的人牙周膜细胞，使用动态机械应变细胞加载仪对细胞进行1%、10%和20%动态牵张应变加载。
METHODS: Primary human periodontal ligament cells were obtained and passaged. Then the cells were stretched by dynamic mechanical strain unit with 1%, 10% and 20% strain at different time.
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