因此本研究选择枯草芽孢杆菌为木聚糖酶基因表达宿主菌。
Therefore, Bacillus subtilis was selected as xylanase gene expression host in this study.
测序表明读码框架正确后,将重组子分别转入表达宿主菌e。
After the reading frame was confirmed correct by sequencing, the recombinant plasmid was transformed into expression host e.
④过表达葡糖胺-6-磷酸合酶对宿主菌细胞壁完整性的影响。
Overexpression glucosamine 6-phosphate synthase effect host strain's Cell wall integrity.
③过表达葡糖胺-6-磷酸合酶对宿主菌抵抗万古霉素的实验;
Overexpression glucosamine 6-phosphate synthase effect host strain's vancomycin-resistant;
此方法可以用于重组溶葡萄球菌酶中残余宿主菌菌体蛋白含量的测定。
The method is simple, sensitive and accurate, so the trace of bacteria protein in recombinant lysostaphin products can be detected.
本文研究了中性蛋白酶基因在不同宿主菌及各种培养条件下的蛋白酶表达水平。
This paper presented the study of expression of neutral protease gene from Bacillus Subtilis in various host cells and different culture conditions.
结论:表达质粒的拷贝数、宿主菌培养条件、细胞破碎方式等均能影响酶的表达量。
Conclusion: The culture conditions and procedure of cell disrepution can influence the expression of GDH.
针对不同宿主菌其噬菌斑形态、大小、透明度、边缘界限、中心透明区及晕环均发生相应变化;
The plaque morphology, size, transparence, central transparent region, and the halo changed according to different host bacteria;
对重组菌中重组质粒的稳定性进行研究,结果表明该质粒在宿主菌中具有良好的分离稳定性,而结构稳定性较差。
The plasmid stability studies showed that recombinant plasmid has excellent segregational stability but the structural stability was not good in the host cell.
从患病南方大口鲶分离得到病原菌N1、N2、N3、N4,以N3为宿主菌,从鱼塘淤泥中分离到三株蛭弧菌。
The pathogenic strains were isolated from sick Silurus meridionalis, named N1, N2, N3 and N4. And three Bdellovibrios were isolated from fishpond sullage using N3 as prey.
摘要 :细菌与噬菌体间是一种寄生关系,一方面细菌抵抗噬菌体感染,另一方面噬菌体也产生抗宿主菌防御的改变。
Abstract :Parasitic relationship exists between bacteria and phages, on one hand, bacteria resist the infection from phages, on another hand, phages develop the abilities against host bacteria.
将构建好的ABP1及TIR1原核表达载体,转化表达宿主菌bl21 (DE3),加入诱导物iptg进行诱导表达。
The ABP1 and TIR1 prokaryotic expression vectors were transformed into expression host strain BL21 (DE3), then after, IPTG was added to induce expression.
除宿主菌的特性及其所含质粒分子量大小之外,消除率还与消除剂浓度、作用时间有关。嵌合染料适用于消除大肠杆菌中的质粒。
Besides the character of host strains and the molecular weight of the plasmid, the efficiency of curing was also related to the concentrations of curing agents and the time of treatment.
脑膜炎奈瑟菌仅感染人类,不存在动物宿主。
Neisseria meningitidis only infects humans; there is no animal reservoir.
根瘤菌通过形成根瘤和它们的宿主建立了一种共生关系——用氮换取营养。 根瘤通常会渗透进植物的根,有时候还会渗透进植物的茎。
Rhizobia establish a symbiotic relationship with their host plant ― exchanging nitrogen for nutrients ― by forming nodules, which penetrate the roots and sometimes stems of the plant.
一项以小鼠为对象的研究发现:肠道菌群的类型影响宿主行为。
A study with mice finds that the types of intestinal bacteria an individual carries can influence behavior. Karen Hopkin reports.
该实验室发现,如果这些蛋白质不能正确排列,沙门氏菌将和其他致病菌一样,不能将自己“注入”宿主细胞并掌控宿主细胞功能。
If the proteins do not line up properly, Salmonella, as well as many other bacterial pathogens, cannot "inject" them into host cells to commandeer host cell functions, the lab has found.
免疫缺陷的宿主中口腔念珠菌病很普通,例如HIV感染。见毛状舌胎,并有浅棕色渗出物,即毛状白斑舌。
Oral candidiasis is common in immunocompromised hosts, such as those with HIV infection. There is a hairy coating of the tongue seen here mixed with a pale tan exudate.
利用异源宿主合成聚酮化合物要比使用天然生产菌有很多优点。
The production of polyketides in heterologous hosts offers many advantages over the use of natural producers.
来自受体菌自身的同源质粒,因克服了宿主的限制一修饰性,可以极显著地提高电转化效率。
A significant increase of transformation efficiency was revcaled with the homologous plasmid isolated from recipient itself since the effects of host restriction and modification werc avoided.
另一种药物,则是造成披衣菌挪用宿主细胞脂质的机制失效,使得这些非法入侵者无法藏匿。
Another drug might incapacitate the mechanisms the bacteria use to divert lipids from the host cell to the chlamydial vacuole, halting the trespassers' ability to hide.
该分泌器横亘囊泡膜层,可做为披衣菌与宿主细胞细胞质之间的通道。
This apparatus, which spans the membrane of the entry vacuole, serves as a conduit between the bacteria and the cytoplasm of the host cell.
结论参四方煎剂治疗慢性顽固性腹泻临床疗效显著,对肠道菌群的消长调节作用明显,能重建宿主的微生态平衡。
Conclusion Shensifang Decoction has obvious therapeutic effect on chronic refractory diarrhea. It can regulate intestinal flora and reestablish micro-ecological balance.
尽管其他吸血虫也许是包柔氏螺旋体菌的中间宿主,但是它们对莱姆病的传播并不起作用。
Though other biting insects may be intermediate hosts forb. Burgdorferi, they were found to have an insignificant role in the spread of lyme disease.
志贺菌在与宿主肠道上皮细胞接触后,激活t3ss并将效应子蛋白注入真核宿主细胞内,引起细菌性痢疾。
Once Shigella makes contact with intestine epithelium, the T3SS is activated and effector proteins are injected into the cytosol of intestine epithelium to cause shigellosis.
方法采用宿主范围测定、噬菌斑计数、负染色电镜观察、血清中和试验及血清交叉中和试验、一步生长实验等技术观察分离的噬菌体。
Methods the phage was identified by detection of the host range, calculation of phage plaque, negative staining for TEM, serological neutralization test and one step growth experiment.
结瘤基因的系统发育揭示出根瘤菌宿主范围与共同性结瘤基因间比其它基荫的相关性更高。
The phylogenetic studies on nod genes have promulgated that the host range of rhizobia appeared to have a higher interrelationship to the common nod genes than other genes.
宿主的炎症性反应有一个关键的破坏胃酸稳态的功能性作用,然后直接形成对感染胃部的HP菌群和胃炎范围的影响。
The host inflammatory response has a key functional role in disrupting acid homeostasis, which impacts directly on the colonization patterns of H pylori and thus the extent of gastritis.
第一部分为筛选具有益生菌特性的乳酸菌,以提供肠道保健及当做第二部分的穿梭载体系统之宿主。
In part I, the probiotic Lactic acid bacteria was screened and used for provide intestinal health and host of shuttle vector of LAB.
第一部分为筛选具有益生菌特性的乳酸菌,以提供肠道保健及当做第二部分的穿梭载体系统之宿主。
In part I, the probiotic Lactic acid bacteria was screened and used for provide intestinal health and host of shuttle vector of LAB.
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