目的探讨实时荧光定量聚合酶链反应(FQ - PCR)在检测外周血清及单核细胞中hcvRNA含量的临床应用价值。
Objective To investigate the clinical application of real time fluorescence quantitive Polymerase Chain Reaction (FQ-PCR) in detection of HCV RNA in serum and peripheral blood monocular cells (PBMC).
实时荧光定量PCR技术是一种利用荧光检测方法来定量核酸的技术，具有高度的灵敏性、特异性和精确性。
Real-time fluorescent quantitative PCR is a kind of technique that can quantify the nucleic acid on different fluorescence, as it features high sensitivity, accuracy, and specificity.
对筛选出的部分差异基因，选取病例组18例及正常对照组6例，以实时荧光定量PCR进行验证。
To filter out some of the differential genes, 18 cases of AS group and 6cases of healthy control group are validated by real-time fluorescence quantitative PCR.
结果建立的实时荧光定量PCR方法的灵敏度为10-4水平。
Results The sensitivity of the established real time quantitative PCR was at 10 -4 level.
目的建立灵敏、稳定、特异的实时荧光PCR方法，用于戊型肝炎病毒的定量检测。
To establish a TaqMan-based real-time fluorescent quantitative PCR assay for detection and quantitation of Hepatitis E virus.
目前常用的PCR法有多重PCR技术、实时荧光定量PCR技术、原位PCR技术及免疫PCR技术，各种技术有不同的原理及优缺点。
At present, the com-mon methods include multiplex PCR, Real Time Quantitative PCR, in situ PCR and immuno-polymerase chain reaction. They have different principles, advantages and disadvantages.
方法将接受拉米夫定治疗的188例患者根据治疗时间进行分组，采用实时荧光PCR方法定量检测各组患者血清HBVDNA水平和酪氨酸-甲硫氨酸-天冬氨酸-天冬氨酸（YMDD）变异。
Methods Serum samples from 188 chronic hepatitis B patients with lamivudine therapy were collected and quantitatively tested with real-time PCR for HBV DNA and YMDD mutations.
目的探讨实时荧光定量pcr检测巨细胞病毒在肾移植术后肺部感染诊断中的应用价值。
Objective to examine the value of real time PCR in the diagnosis of cytomegalovirus pneumonia in renal Allograft Recipient.
目的建立一种快速、灵敏、特异的鉴定克柔念珠菌和光滑念珠菌的双重实时荧光定量PCR方法。
Objective To set up a duplex quantitative real-time PCR (QPCR) assay with high sensitivity, specificity and rapidity to detect Candida krusei and Candida glabrata.
构建汉坦病毒S片段标准品，用于实时荧光定量pcr检测汉坦病毒。
To explore the method of preparation for Hantavirus s segment standard plasmids of real-time PCR.
文章对实时荧光定量pcr的原理、影响因素以及在植物病害和遗传育种方面的应用等进行了综述。
Article on the principle of real-time fluorescent quantitative PCR, factors and plant diseases and genetic breeding of the application were reviewed.
采用实时荧光定量pcr的方法进行定量分析。
Uses real-time fluorescence quota PCR the method to carry on the quantitative analysis.
通过表达谱芯片检测药物作用后的基因表达差异，对差异表达基因进行生物信息学研究并结合实时荧光定量pcr分析。
Then the GO and KEGG Pathway enrichment analysis were carried as well as the functional analysis and real time RT-PCR validation of two important genes.
免疫荧光法和实时定量PCR有较好的一致性（92.3%）。
The correspondence of the HCMV pp65 test and quantitative PCR was 92.3%.
方法采用实时荧光定量PCR检测113个腓骨肌萎缩症家系先证者、4个遗传性压力易感性神经病家系先证者和50名正常人PMP22基因重复或缺失突变。
Methods Duplications or deletions of?PMP22gene were detected in 113 CMT cases, 4 HNPP cases and 50 normal controls by using real-time quantitative PCR. Results (Thirty-six) of 113 CMT cases had the?
实时荧光定量PCR结果表明，该基因在受到条锈菌诱导下，在亲和组合中表达趋势明显下调，而在非亲合组合中的表达趋势比较稳定。
The results of real-time PCR analysis revealed that TAFFC transcripts were steady in incompatible interaction of wheat and stripe rust, whereas down-regulated in compatible interaction.
结果表明，建立的实时荧光定量pcr方法特异性强、重复性好，为定量检测鸡细胞因子的一种快速、准确的方法。
The results showed that the established QRT-PCR was specific and reproducible, and could be used for the rapid and accurate detection of chicken cytokines.
采用实时荧光定量PCR鉴定高表达基因。
Differentially expressed genes were identified by real-time quantitative PCR.
实时荧光定量PCR试验表明，FABP4基因在160和200日龄绵羊背最长肌中的表达量明显高于90日龄背最长肌的表达量（P< 0.05）；
The real time RT-PCR revealed that expressions of FABP4 in longissimus muscle from 160 and 200-day's sheep were higher than those from 90-day's (P<0.05), expectively.
实时荧光定量pcr广泛应用于植物病理学研究的各个领域。
Real time quantitative PCR has been widely used in various areas of plant pathology research.
我们对部分细胞周期、细胞凋亡、细胞应激与炎症反应相关基因进行了分析并用实时荧光定量pcr进行了验证，证实了基因芯片结果的可靠性。
We analyze some genes including cell cycle, apoptosis, stress and inflammation, which are confirmed by the real time RT-PCR, and the results are corresponded to that of cDNA microarray.
方法采用实时荧光定量pcr、ELISA及速率法分别检测60例乙肝患者经拉米呋啶治疗后其血清YMDD、HBVDNA、乙肝标志物及alt的变化情况。
Methods the serum YMDD mutation, HBV DNA, ALT and HbeAg levels of 60 patients on lamivudine therapy were detected by FQ-PCR, ELISA, and rate method, respectively.
方法采用实时荧光定量聚合酶链反应和免疫组化染色法检测96例食管癌患者的食管癌组织和癌旁正常组织Caspase-8表达。
Methods We quantified Caspase-8 expression levels in 96 esophageal cancer issues and their matched normal esophagus tissues by using real-time PCR assay and immunohistochemistry staining.
方法采用实时荧光定量聚合酶链反应和免疫组化染色法检测96例食管癌患者的食管癌组织和癌旁正常组织Caspase-8表达。
Methods We quantified Caspase-8 expression levels in 96 esophageal cancer issues and their matched normal esophagus tissues by using real-time PCR assay and immunohistochemistry staining.
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