方法应用实时监测聚合酶链反应检测这三种受体基因在雌激素受体阳性和雌激素受体阴性乳腺癌细胞系中的表达。
Methods These three genes were quantified by real-time quantification polymerase chain reaction (PCR) in er positive and er negative breast cancer cell lines.
目的探讨实时荧光定量聚合酶链反应(FQ - PCR)在检测外周血清及单核细胞中hcvRNA含量的临床应用价值。
Objective To investigate the clinical application of real time fluorescence quantitive Polymerase Chain Reaction (FQ-PCR) in detection of HCV RNA in serum and peripheral blood monocular cells (PBMC).
经实时荧光定量逆转录聚合酶链反应验证了基因芯片准确可靠。
The results of realtime RTPCR proved the accuracy of the gene chip.
方法采用实时荧光定量聚合酶链反应和免疫组化染色法检测96例食管癌患者的食管癌组织和癌旁正常组织Caspase-8表达。
Methods We quantified Caspase-8 expression levels in 96 esophageal cancer issues and their matched normal esophagus tissues by using real-time PCR assay and immunohistochemistry staining.
方法采用实时荧光定量聚合酶链反应和免疫组化染色法检测96例食管癌患者的食管癌组织和癌旁正常组织Caspase-8表达。
Methods We quantified Caspase-8 expression levels in 96 esophageal cancer issues and their matched normal esophagus tissues by using real-time PCR assay and immunohistochemistry staining.
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