最后，用定量实时PCR法在一组新样本(20名发作性睡病-猝倒患者和20名健康对照)中进一步确认候选基因的表达水平。
Finally, the expression levels of the candidate genes were further confirmed by quantitative real-time PCR using a new set of samples (20 narcolepsy-cataplexy patients and 20 healthy controls).
实时荧光定量PCR技术是一种利用荧光检测方法来定量核酸的技术，具有高度的灵敏性、特异性和精确性。
Real-time fluorescent quantitative PCR is a kind of technique that can quantify the nucleic acid on different fluorescence, as it features high sensitivity, accuracy, and specificity.
目的运用实时P CR技术，对未获培养微生物au126与牙周病的关系进行定量分析。
Objective To quantitatively analyse the relationship between uncultivated phylotype AU126 and periodontal disease with real-time PCR.
目的探讨实时荧光定量聚合酶链反应(FQ - PCR)在检测外周血清及单核细胞中hcvRNA含量的临床应用价值。
Objective To investigate the clinical application of real time fluorescence quantitive Polymerase Chain Reaction (FQ-PCR) in detection of HCV RNA in serum and peripheral blood monocular cells (PBMC).
目前常用的PCR法有多重PCR技术、实时荧光定量PCR技术、原位PCR技术及免疫PCR技术，各种技术有不同的原理及优缺点。
At present, the com-mon methods include multiplex PCR, Real Time Quantitative PCR, in situ PCR and immuno-polymerase chain reaction. They have different principles, advantages and disadvantages.
方法将接受拉米夫定治疗的188例患者根据治疗时间进行分组，采用实时荧光PCR方法定量检测各组患者血清HBVDNA水平和酪氨酸-甲硫氨酸-天冬氨酸-天冬氨酸（YMDD）变异。
Methods Serum samples from 188 chronic hepatitis B patients with lamivudine therapy were collected and quantitatively tested with real-time PCR for HBV DNA and YMDD mutations.
目的建立一种快速、灵敏、特异的鉴定克柔念珠菌和光滑念珠菌的双重实时荧光定量PCR方法。
Objective To set up a duplex quantitative real-time PCR (QPCR) assay with high sensitivity, specificity and rapidity to detect Candida krusei and Candida glabrata.
所用的DNA方法学和所处的妊娠期对检测精度有着最大的影响，实时定量多聚酶链反应(RTQ -PCR)的检测精度超过常规的PCR。
DNA methodology and gestational age had the largest effects on test performance, with real-time quantitative polymerase chain reaction (RTQ-PCR) outperforming conventional PCR.
结果建立的实时荧光定量PCR方法的灵敏度为10-4水平。
Results The sensitivity of the established real time quantitative PCR was at 10 -4 level.
对筛选出的部分差异基因，选取病例组18例及正常对照组6例，以实时荧光定量PCR进行验证。
To filter out some of the differential genes, 18 cases of AS group and 6cases of healthy control group are validated by real-time fluorescence quantitative PCR.
目的探讨实时荧光定量pcr检测巨细胞病毒在肾移植术后肺部感染诊断中的应用价值。
Objective to examine the value of real time PCR in the diagnosis of cytomegalovirus pneumonia in renal Allograft Recipient.
方法利用寡核苷酸基因芯片技术和实时定量pcr技术检测20例胰腺癌组织和6例正常胰腺组织中TUSC3基因的表达。
Methods The expression of TUSC3 gene in 20 cases of pancreatic cancer and 6 cases of normal pancreas were detected by oligonucleotide microarray and real-time quantitative PCR.
构建汉坦病毒S片段标准品，用于实时荧光定量pcr检测汉坦病毒。
To explore the method of preparation for Hantavirus s segment standard plasmids of real-time PCR.
目的建立灵敏、稳定、特异的实时荧光PCR方法，用于戊型肝炎病毒的定量检测。
To establish a TaqMan-based real-time fluorescent quantitative PCR assay for detection and quantitation of Hepatitis E virus.
用实时定量pcr检测标本线粒体dna的拷贝数。
Moreover, to detect the mitochondria DNA copy Numbers of sample with real-time PCR.
免疫荧光法和实时定量PCR有较好的一致性（92.3%）。
The correspondence of the HCMV pp65 test and quantitative PCR was 92.3%.
通过表达谱芯片检测药物作用后的基因表达差异，对差异表达基因进行生物信息学研究并结合实时荧光定量pcr分析。
Then the GO and KEGG Pathway enrichment analysis were carried as well as the functional analysis and real time RT-PCR validation of two important genes.
文章对实时荧光定量pcr的原理、影响因素以及在植物病害和遗传育种方面的应用等进行了综述。
Article on the principle of real-time fluorescent quantitative PCR, factors and plant diseases and genetic breeding of the application were reviewed.
方法采用实时荧光定量PCR检测113个腓骨肌萎缩症家系先证者、4个遗传性压力易感性神经病家系先证者和50名正常人PMP22基因重复或缺失突变。
Methods Duplications or deletions of?PMP22gene were detected in 113 CMT cases, 4 HNPP cases and 50 normal controls by using real-time quantitative PCR. Results (Thirty-six) of 113 CMT cases had the?
实时荧光定量PCR结果表明，该基因在受到条锈菌诱导下，在亲和组合中表达趋势明显下调，而在非亲合组合中的表达趋势比较稳定。
The results of real-time PCR analysis revealed that TAFFC transcripts were steady in incompatible interaction of wheat and stripe rust, whereas down-regulated in compatible interaction.
实时荧光定量PCR试验表明，FABP4基因在160和200日龄绵羊背最长肌中的表达量明显高于90日龄背最长肌的表达量（P< 0.05）；
The real time RT-PCR revealed that expressions of FABP4 in longissimus muscle from 160 and 200-day's sheep were higher than those from 90-day's (P<0.05), expectively.
进行实时定量PCR反应，建立标准曲线；
Real-time PCR reaction; Amplification curve output; Ct data obtain;
采用实时荧光定量pcr的方法进行定量分析。
Uses real-time fluorescence quota PCR the method to carry on the quantitative analysis.
采用实时荧光定量PCR鉴定高表达基因。
Differentially expressed genes were identified by real-time quantitative PCR.
结果表明，建立的实时荧光定量pcr方法特异性强、重复性好，为定量检测鸡细胞因子的一种快速、准确的方法。
The results showed that the established QRT-PCR was specific and reproducible, and could be used for the rapid and accurate detection of chicken cytokines.
利用多重PCR和实时定量PCR技术可以实现对葡萄酒中生物胺产生菌的快速定量检测。
Multiplex PCR and real-time PCR are developed for simultaneous and quantitative detection of bacteria-producing biogenic amine.
实时荧光定量pcr广泛应用于植物病理学研究的各个领域。
Real time quantitative PCR has been widely used in various areas of plant pathology research.
采用实时定量pcr (RT - PCR)检测正常及糖尿病大鼠心肌组织ace2的基因表达。
Myocardial ACE2 mRNA expression was respectively determined by real-time PCR (RT-PCR) in control group and diabetes group.
方法采用实时荧光定量聚合酶链反应和免疫组化染色法检测96例食管癌患者的食管癌组织和癌旁正常组织Caspase-8表达。
Methods We quantified Caspase-8 expression levels in 96 esophageal cancer issues and their matched normal esophagus tissues by using real-time PCR assay and immunohistochemistry staining.
方法采用实时荧光定量聚合酶链反应和免疫组化染色法检测96例食管癌患者的食管癌组织和癌旁正常组织Caspase-8表达。
Methods We quantified Caspase-8 expression levels in 96 esophageal cancer issues and their matched normal esophagus tissues by using real-time PCR assay and immunohistochemistry staining.
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