进行实时定量PCR反应，建立标准曲线；
Real-time PCR reaction; Amplification curve output; Ct data obtain;
用实时定量pcr检测标本线粒体dna的拷贝数。
Moreover, to detect the mitochondria DNA copy Numbers of sample with real-time PCR.
免疫荧光法和实时定量PCR有较好的一致性（92.3%）。
The correspondence of the HCMV pp65 test and quantitative PCR was 92.3%.
实时定量的监测气侵，一直是安全钻井特别是气井作业的重要课题。
It is important to detect and measure gas cutting at real time and quantitatively, specially during gas well production.
结论实时定量pcr技术是验证sage等高通量数据的可靠工具之一。
Conclusions Real-time PCR is a reliable tool for the validation of high through-put data such as SAGE.
利用多重PCR和实时定量PCR技术可以实现对葡萄酒中生物胺产生菌的快速定量检测。
Multiplex PCR and real-time PCR are developed for simultaneous and quantitative detection of bacteria-producing biogenic amine.
采用实时定量pcr (RT - PCR)检测正常及糖尿病大鼠心肌组织ace2的基因表达。
Myocardial ACE2 mRNA expression was respectively determined by real-time PCR (RT-PCR) in control group and diabetes group.
方法利用寡核苷酸基因芯片技术和实时定量pcr技术检测20例胰腺癌组织和6例正常胰腺组织中TUSC3基因的表达。
Methods The expression of TUSC3 gene in 20 cases of pancreatic cancer and 6 cases of normal pancreas were detected by oligonucleotide microarray and real-time quantitative PCR.
所用的DNA方法学和所处的妊娠期对检测精度有着最大的影响，实时定量多聚酶链反应(RTQ -PCR)的检测精度超过常规的PCR。
DNA methodology and gestational age had the largest effects on test performance, with real-time quantitative polymerase chain reaction (RTQ-PCR) outperforming conventional PCR.
结论实时心肌声学造影可定量分析x综合征患者心肌微循环灌注情况。
Conclusion myocardial microcirculation in syndrome X could be quantitative analyzed by real-time myocardial contrast echocardiography.
最后，用定量实时PCR法在一组新样本(20名发作性睡病-猝倒患者和20名健康对照)中进一步确认候选基因的表达水平。
Finally, the expression levels of the candidate genes were further confirmed by quantitative real-time PCR using a new set of samples (20 narcolepsy-cataplexy patients and 20 healthy controls).
该系统可以实时、在线、定量的检测钢丝绳的损伤，对损伤的判定较目前的手段更为科学、准确、合理。
The real time, on-line, quantitative testing is offered for system of wire rope destruction, the method of determinant for wire rope destruction is better scientific, nicety, reasonable.
实时荧光定量PCR技术是一种利用荧光检测方法来定量核酸的技术，具有高度的灵敏性、特异性和精确性。
Real-time fluorescent quantitative PCR is a kind of technique that can quantify the nucleic acid on different fluorescence, as it features high sensitivity, accuracy, and specificity.
一种定量实时多聚酶链反应能够快速准确的诊断巨细胞病毒，改善免疫抑制患者的治疗。
A quantitative real-time polymerase chain reaction test provides rapid and accurate diagnosis of cytomegalovirus and improved management of immunocompromised patients.
目的：探讨实时心肌声学造影定量评价犬正常心肌灌注的可行性。
Objective: To assess the value of Real time myocardial contrast echocardiography (MCE) in the quantitative evaluation of canine normal myocardial perfusion.
目的运用实时P CR技术，对未获培养微生物au126与牙周病的关系进行定量分析。
Objective To quantitatively analyse the relationship between uncultivated phylotype AU126 and periodontal disease with real-time PCR.
为实现机械构件线应变的非接触式实时在线定量检测提供新的技术与方法。
Thus new technology and method for realizing non-contacting real-timed on line quantitative detection on linear strain of mechanical components were provided.
目的探讨实时荧光定量聚合酶链反应(FQ - PCR)在检测外周血清及单核细胞中hcvRNA含量的临床应用价值。
Objective To investigate the clinical application of real time fluorescence quantitive Polymerase Chain Reaction (FQ-PCR) in detection of HCV RNA in serum and peripheral blood monocular cells (PBMC).
在对模糊定性仿真算法改进的基础上，提出了一类信息不完整动态系统的实时半定量故障预报方法。
Based on improvement of fuzzy qualitative simulation algorithm, a new semi-quantitative and real-time fault prediction method of a class of dynamic system with incomplete information is proposed.
目的为实现夹板局部外固定治疗骨折的定量化，我们研制了盘状指针式拉力传感器以便实时监控夹板固定带约束力。
ObjectiveIn order to quantify the treatment of fractures with splint, we develop discal index drawing force transducer for measuring and controlling the sanction of fixation retainer of the splint.
结论实时声学造影及GAD能够定量分析冠心病患者心肌血流量情况。
Conclusions Real time contrast echocardiography and GAD can evaluate MBF in patients with CAD correctly and directly.
目前常用的PCR法有多重PCR技术、实时荧光定量PCR技术、原位PCR技术及免疫PCR技术，各种技术有不同的原理及优缺点。
At present, the com-mon methods include multiplex PCR, Real Time Quantitative PCR, in situ PCR and immuno-polymerase chain reaction. They have different principles, advantages and disadvantages.
提出了一种新的投影云纹偏转法用于实时显示和定量测量位相物体的全场密度和温度。
This paper presents a novel method based on projection moire deflectometry for the visualization and quantitative evaluation of full-field density and temperature variations in phase object.
方法将接受拉米夫定治疗的188例患者根据治疗时间进行分组，采用实时荧光PCR方法定量检测各组患者血清HBVDNA水平和酪氨酸-甲硫氨酸-天冬氨酸-天冬氨酸（YMDD）变异。
Methods Serum samples from 188 chronic hepatitis B patients with lamivudine therapy were collected and quantitatively tested with real-time PCR for HBV DNA and YMDD mutations.
为了更好地定量分析可预测性，需要为以固定优先级方式调度的、周期性任务的硬实时CORBA系统建模。
In order to achieve this, scheduling models should be set up for hard real-time CORBA systems with static priority and periodic tasks.
目的建立一种快速、灵敏、特异的鉴定克柔念珠菌和光滑念珠菌的双重实时荧光定量PCR方法。
Objective To set up a duplex quantitative real-time PCR (QPCR) assay with high sensitivity, specificity and rapidity to detect Candida krusei and Candida glabrata.
结果建立的实时荧光定量PCR方法的灵敏度为10-4水平。
Results The sensitivity of the established real time quantitative PCR was at 10 -4 level.
所以，对安全监测信息的使用是安全监测系统应用的主要瓶颈，需要研究如何利用安全监测信息对结构的安全状态进行实时和定量的评估。
Thus the evaluation of the safety of the structure, based on the monitoring information, is a bottleneck of the development of the monitoring system.
超声生物显微镜（UBM）是一种专供眼前段检查的新型超声设备，具有简便、实时、无创等特点，不仅可清楚显示眼前段形态结构，并且可进行定量检测。
Ultrasound Biomicroscopy(UBM)is a new ultrasonic equipment with high-frequency transducer by which structures in the anterior part of eyes can be real-time observed and measured exactly.
文章对实时荧光定量pcr的原理、影响因素以及在植物病害和遗传育种方面的应用等进行了综述。
Article on the principle of real-time fluorescent quantitative PCR, factors and plant diseases and genetic breeding of the application were reviewed.
应用推荐