方法取大鼠胚胎分离成纤维细胞，分别采用细胞悬液法和胰酶消化植块培养法对其进行培养。
Methods Separate the embryonic fibroblast of mice and culture with the method of suspension culture and method of trypsinization.
通过比较LIF和大鼠心肌条件液在小鼠es细胞分离培养过程中的差异，从而选择较为合适的培养液用于小鼠es细胞的分离培养与深入研究。
By comparing the difference between LIF media conditioned and rat cardiomyocyte media conditioned during isolation and culture of mouse ES cell, suitable media is selected in the future.
方法：采用胶原酶灌注法及剪碎消化法分离大鼠胎肝干细胞，并用含10%优等胎牛血清的H -DMEM培养液培养。
Methods Collagenase perfusion method and mechanical cutting method were used to isolate HSCs from rat fetal liver which were then cultivated by H-DMEM containing 10% fetal bovine serum.
方法利用盐酸利多卡因注射液联合震荡培养法分离纯化胎大鼠脑内小胶质细胞，免疫荧光技术及流式细胞技术鉴定小胶质细胞的纯度。
Method: Fetal rat microglia in brain was isolated and purified by lidocaine hydrochloride with shock culturing. The purification of microglia was detected by immunofluorescence and flow cytometry.
方法利用盐酸利多卡因注射液联合震荡培养法分离纯化胎大鼠脑内小胶质细胞，免疫荧光技术及流式细胞技术鉴定小胶质细胞的纯度。
Method: Fetal rat microglia in brain was isolated and purified by lidocaine hydrochloride with shock culturing. The purification of microglia was detected by immunofluorescence and flow cytometry.
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