目的构建可用于大肠杆菌表达系统的含抗冻蛋白基因afp的表达载体。
Objective: to obtain the procaryotic expression vector containing the antifreeze protein gene AFP.
结论成功用大肠杆菌表达系统表达创伤弧菌溶细胞素并对其纯化、复性条件进行优化。
These results indicate that the cytolysin gene from V. vulnificus successfully express in the E. coli system.
创伤弧菌中的溶细胞素在大肠杆菌系统中成功表达。
The cytolysin gene from V. vulnificus successfully express in the E. coli system.
结论：用硫氧还蛋白融合表达系统在大肠杆菌中表达的小鼠内皮抑素重组融合蛋白易纯化并具有高活性。
CONCLUSION: The mouse endostatin recombinant fusion protein expressed in E. coli by using thioredoxin fusion expression system is easy to be purified and possesses high activity.
用融合蛋白表达系统对该半分子进行大肠杆菌表达。
Then, a fusion protein expression system was used to express the TNF half molecule.
枯草芽孢杆菌是继大肠杆菌之后又一广泛使用的表达系统，它没有致病性，只具有单层细胞外膜，并且能直接将许多蛋白分泌到培养基中。
As another widely used expression system following E. coli, Bacillus subtilis is not pathogenic with single-cell membrane and secreted proteins to medium directly.
枯草芽孢杆菌是继大肠杆菌之后又一广泛使用的表达系统，它没有致病性，只具有单层细胞外膜，并且能直接将许多蛋白分泌到培养基中。
As another widely used expression system following E. coli, Bacillus subtilis is not pathogenic with single-cell membrane and secreted proteins to medium directly.
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