方法应用多重PCR技术检测63例肝癌患者和88例健康对照的GSTM1和GSTT1空白基因型。
Methods The genotypes of GSTM1 and GSTT1 of 63 cases with HCC and 88 controls were detected with multiple PCR technique.
方法在140名汉族健康人的外周血中，应用聚合酶链反应（PCR）限制性片段长度多态性分析（RFLP）及多重PCR技术，进行NAT1等位基因分型研究。
Methods Using multiple PCR and PCR-RFLP methods, we studied the NAT1 genotypes and its genetic polymorphisms of the peripheral blood samples from 140 Han people.
方法：采用多重聚合酶链反应方法，以提取的基因组d NA为模板，在同一反应管中同时扩增细胞周期素d 1基因和P 16基因。
METHODS: Method of multiplex polymerase chain reaction (PCR) was adopted. Cyclin D1 gene and P16 gene were amplified in the same tube, using extracted genome DNA as template.
建立了一种同时检测猪口蹄疫病毒(FMDV)、猪水泡病病毒(SVDV)和猪水疱性口炎病毒(VSV)三种病原体的多重rt - PCR方法。
A multiplex RT-PCR was optimized to simultaneously detect foot-and-mouth disease virus (FMDV), swine vesicular disease virus (SVDV) and vesicular stomatitis virus (VSV).
多重PCR方法是建立在传统PCR检测技术基础上的快速简便的检测手段。
The multiplex PCR is a progressing rapid detection method developed from traditional PCR detection technique.
前言：目的建立适于基层单位应用的针对副溶血性弧菌流行群的特异多重PCR方法。
Objective In order to set up rapid identification for the pandemic vibrio parahaemolyticus, develope a pandemic group-specific multiplex PCR assay for base unit.
设计和搜索多重引物，包括PCR引物、序列探针和测序引物。
Design and search primers for multiple applications including PCR, DNA hybridization and sequencing.
结果运用群特异多重PCR法可特异性的扩增出当前流行群副溶血性弧菌的目的基因片段，可大大缩短鉴定时间。
Results The aim gene fragment can be amplified by a multiplex PCR assay, it largely reduce the time of identification.
结果表明：多重pcr技术可成功应用于SARS病毒的检测。
The results indicated that the multiplex PCR technique could be applied to detect the SARS virus, successfully.
目前常用的PCR法有多重PCR技术、实时荧光定量PCR技术、原位PCR技术及免疫PCR技术，各种技术有不同的原理及优缺点。
At present, the com-mon methods include multiplex PCR, Real Time Quantitative PCR, in situ PCR and immuno-polymerase chain reaction. They have different principles, advantages and disadvantages.
我们建立了1种易于使用的于含15种染色体微卫星标志的多重PCR中对鼠弓形虫进行分离株基因分型的方法。
We developed an easy-to-use method for genotyping Toxoplasma gondii isolates in a single multiplex PCR assay with 15 microsatellite markers.
同时分析了STR多态性与多重等位基因特异pcr (MASPCR)在PKU基因诊断中的联合应用。
United application of STR linkage analysis and multiplex allele specific PCR (MASPCR) in PKU genetic diagnosis was also analysed.
目的：建立先天致畸多种病原体(TORCH)感染的多重pcr诊断方法。
Objective: TO establish a multiple polymerase chain reaction (multiplex PCR) technique for detection of TORCH infection.
结果表明，建立的多重pcr方法能够准确的同时检测出豆粕中的内源基因和转基因成分。
The results showed that the high efficiency PCR system can differentially, conveniently, effectively and coinstantaneously examine the endogenous gene Lectin and the transgenic components.
目的了解采用双纸片协同法进行CTX - M酶的表型检测与多重pcr法检测基因型的相关性。
Objective to investigate the correlation between phenotypic confirmatory by double disc test and genotype of CTX-M-enzymes detected by multiplex polymerase chain reaction (PCR).
摘要 ：目的基于多重聚合酶链反应（PCR）建立一种快速方便的检测副溶血弧菌“大流行菌群”及其毒力基因的方法。
Abstract : Objective To establish a multiplex PCR assay for the rapid detection of pandemic group of Vibrio parahaemolyticus and toxic genes based on GS-PCR.
多重PCR法扩增待杂交并于芯片上微型测序的靶DNA。
The DNA reference targets for hybridization and subsequent on chip minisequencing was generated by multiplex PCR.
应用三对具有特异性的寡核苷酸引物，进行多重pcr扩增。
Three pairs of oligonucleotide primer were used in triplex-PCR.
利用多重PCR和实时定量PCR技术可以实现对葡萄酒中生物胺产生菌的快速定量检测。
Multiplex PCR and real-time PCR are developed for simultaneous and quantitative detection of bacteria-producing biogenic amine.
方法应用三对具有特异性的寡核普酸引物，进行多重PCR扩增。
Method three pairs of oligonucleotide primer were used in triplex-PCR.
应用聚合酶链式反应（PCR）、多重PCR方法检测GSTM1和GSTT1在正常人群和结直肠肿瘤患者群体中的基因多态性分布。
The frequencies of GSTM1-null and GSTT1-null genotypes in colorectal tumor and control groups were ascertained by using polymerase chain reaction (PCR) and multiple-PCR techniques.
它不但能进行常规PCR引物、杂交探针和测序引物的设计，同时，它还能进行多重pcr引物分析以及高通量的引物搜索和查找功能。
In addition to supporting conventional applications for PCR, hybridization and sequencing, NoePrimer provides advanced capabilities for multiplex PCR and high-throughput primer search and analysis.
多重pcr作为一种可同时检测多种病原细菌的方法应用日益广泛。
Multiplex PCR which can simultaneously detect more than one bacteria has been applied increasingly and widely.
方法：采用多重pcr核酸探针杂交技术，对泌尿生殖系统传播疾病(STD)有症状者分泌物，用同一份处理标本与常规pcr法比较。
Methods: with multi-PCR-nucleic-acid probe, We tested the secretion specimens from STD patients with clinical symptoms and compared the results with those of the routine PCR.
有学者建立了检测prrsv、PCV2和CSFV的PCR技术，但同时检测这三种病毒的多重pcr方法还未见报道。
Some scholars have established a PCR for testing PRRSV, PCV2 and CSFV, but the multiplex PCR for the three viruses is none.
进而说明该研究建立的大肠杆菌、沙门氏菌和葡萄球菌多重pcr检测方法敏感性高、特异性强，适用于大肠杆菌、沙门氏菌和葡萄球菌的流行病学调查研究。
Then this multiple PCR indicated that it was fit for the studies on the epidemiology investigation of E. coli, Salmonella bacillus and Staphylococcus which has good sensitivity and Specificity.
目的建立可同时进行艰难梭菌分离株菌种鉴定和毒素检测的多重pcr方法。
Objective to design a multiplex PCR for simultaneous identification and toxigenic type characterization of Clostridium difficile isolates.
方法 对62例无精症、少精症患者及20例正常男性采用多重聚合酶链反应法进行AZF区基因微缺失检测。
Methods PCR method was used to detect micro-deletion in AZF gene in 62 oligospermatism and azoospermatism patients and 20 normal male controls.
结果巢式多重PCR检测体系可以检测出单拷贝质粒DNA。
Results One DNA copy of each gene could be detected with our multiplex nested PCR system.
结果巢式多重PCR检测体系可以检测出单拷贝质粒DNA。
Results One DNA copy of each gene could be detected with our multiplex nested PCR system.
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