方法 对62例无精症、少精症患者及20例正常男性采用多重聚合酶链反应法进行AZF区基因微缺失检测。
Methods PCR method was used to detect micro-deletion in AZF gene in 62 oligospermatism and azoospermatism patients and 20 normal male controls.
摘要 ：目的基于多重聚合酶链反应（PCR）建立一种快速方便的检测副溶血弧菌“大流行菌群”及其毒力基因的方法。
Abstract : Objective To establish a multiplex PCR assay for the rapid detection of pandemic group of Vibrio parahaemolyticus and toxic genes based on GS-PCR.
方法：采用多重聚合酶链反应方法，以提取的基因组d NA为模板，在同一反应管中同时扩增细胞周期素d 1基因和P 16基因。
METHODS: Method of multiplex polymerase chain reaction (PCR) was adopted. Cyclin D1 gene and P16 gene were amplified in the same tube, using extracted genome DNA as template.
方法：采用多重聚合酶链反应方法，以提取的基因组d NA为模板，在同一反应管中同时扩增细胞周期素d 1基因和P 16基因。
METHODS: Method of multiplex polymerase chain reaction (PCR) was adopted. Cyclin D1 gene and P16 gene were amplified in the same tube, using extracted genome DNA as template.
应用推荐